MAPK6 monoclonal antibody (M01), clone 1G6
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of MAPK6 expression in transfected 293T cell line by MAPK6 monoclonal antibody (M01), clone 1G6.
Lane 1: MAPK6 transfected lysate(82.7 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MAPK6 is approximately 0.03ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of MAPK6 over-expressed 293 cell line, cotransfected with MAPK6 Validated Chimera RNAi ( Cat # H00005597-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK6 monoclonal antibody (M01) clone 1G6 (Cat # H00005597-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunofluorescence
Immunofluorescence of monoclonal antibody to MAPK6 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (37.84 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant MAPK6.
Immunogen
MAPK6 (AAH35492, 612 a.a. ~ 721 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
EVRKDEQVEKENTYTSYLDKFFSRKEDTEMLETEPVEDGKLGERGHEEGFLNNSGEFLFNKQLESIGIPQFHSPVGSPLKSIQATLTPSAMKSSPQIPHQTYSSILKHLN
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (86); Rat (85)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of MAPK6 expression in transfected 293T cell line by MAPK6 monoclonal antibody (M01), clone 1G6.
Lane 1: MAPK6 transfected lysate(82.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MAPK6 is approximately 0.03ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of MAPK6 over-expressed 293 cell line, cotransfected with MAPK6 Validated Chimera RNAi ( Cat # H00005597-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK6 monoclonal antibody (M01) clone 1G6 (Cat # H00005597-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to MAPK6 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — MAPK6
Entrez GeneID
5597GeneBank Accession#
BC035492Protein Accession#
AAH35492Gene Name
MAPK6
Gene Alias
DKFZp686F03189, ERK3, HsT17250, PRKM6, p97MAPK
Gene Description
mitogen-activated protein kinase 6
Omim ID
602904Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq
Other Designations
MAP kinase isoform p97|extracellular signal-regulated kinase 3|extracellular signal-regulated kinase, p97|protein kinase, mitogen-activated 5|protein kinase, mitogen-activated 6
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Interactome
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Pathway
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