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PRF1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005551-T02
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-PRF1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 61.4
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-PRF1 antibody (H00005551-B01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00005551-T02
    PRF1 transfected lysate.
    Western Blot
    QC Testing of H00005551-T02
    Lane 1: PRF1 transfected lysate ( 61.40 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5551
  • Gene Name:
  • PRF1
  • Gene Alias:
  • FLH2,HPLH2,MGC65093,P1,PFN1,PFP
  • Gene Description:
  • perforin 1 (pore forming protein)
  • Gene Summary:
  • The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000019759,cytolysin,lymphocyte pore forming protein,perforin 1
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