PPID monoclonal antibody (M01), clone 4C7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PPID expression in transfected 293T cell line by PPID monoclonal antibody (M01), clone 4C7.
Lane 1: PPID transfected lysate (Predicted MW: 40.8 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PPID is 3 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to PPID on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (67.2 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant PPID.
Immunogen
PPID (NP_005029.1, 1 a.a. ~ 370 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSHPSPQAKPSNPSNPRVFFDVDIGGERVGRIVLELFADIVPKTAENFRALCTGEKGIGHTTGKPLHFKGCPFHRIIKKFMIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDREGLLSMANAGRNTNGSQFFITTVPTPHLDGKHVVFGQVIKGIGVARILENVEVKGEKPAKLCVIAECGELKEGDDGGIFPKDGSGDSHPDFPEDADIDLKDVDKILLITEDLKNIGNTFFKSQNWEMAIKKYAEVLRYVDSSKAVIETADRAKLQPIALSCVLNIGACKLKMSNWQGAIDSCLEALELDPSNTKALYRRAQGWQGLKEYDQALADLKKAQGIAPEDKAIQAELLKVKQKIKAQKDKEKAVYAKMFA
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (67.2 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PPID expression in transfected 293T cell line by PPID monoclonal antibody (M01), clone 4C7.
Lane 1: PPID transfected lysate (Predicted MW: 40.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PPID is 3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PPID on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — PPID
Entrez GeneID
5481GeneBank Accession#
NM_005038.2Protein Accession#
NP_005029.1Gene Name
PPID
Gene Alias
CYP-40, CYPD, MGC33096
Gene Description
peptidylprolyl isomerase D
Omim ID
601753Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A. [provided by RefSeq
Other Designations
40 kDa peptidyl-prolyl cis-trans isomerase D|PPIase|cyclophilin 40|cyclophilin D|cyclophilin-related protein|rotamase
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Interactome
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Pathway
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Publication Reference
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Levels of 17β-hydroxysteroid dehydrogenase type 10 in CSF are not a valuable biomarker for multiple sclerosis.
Kristofikova Z, Ricny J, Kaping D, Klaschka J, Kotoucova J, Bartos A.
Biomarkers in Medicine 2018 Dec; [Epub].
Application：ELISA, Human, Cerebrospinal fluid from patients with multiple sclerosis.
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Levels of 17β-hydroxysteroid dehydrogenase type 10 in CSF are not a valuable biomarker for multiple sclerosis.
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