POU2F1 (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human POU2F1 full-length ORF ( AAH01664, 1 a.a. - 743 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MNNPSETSKPSMESGDGNTGTQTNGLDFQKRPVPVGGAISTAQAQAFLGHLHQVQLAGTSLQAAAQSLNVQSKSNEESGDSQQPSQPSQQPSVQAAIPQTQLMLAGGQITGLTLTPAQQQLLLQQAQAQAQLLAAAVQQHSASQQHSAAGATISASAATPMTQIPLSQPIQIAQDLQQLQQLQQQNLNLQQFVLVHPTTNLQPAQFIISQTPQGQQGLLQAQNLLTQLPQQSQANLLQSQPSITLTSQPATPTRTIAATPIQTLPQSQSTPKRIDTPSLEEPSDLEELEQFAKTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLEKWLNDAENLSSDSSLSSPSALNSPGIEGLSRRRKKRTSIETNIRVALEKSFLENQKPTSEEITMIADQLNMEKEVIRVWFCNRRQKEKRINPPSSGGTSSSPIKAIFPSPTSLVATTPSLVTSSAATTLTVSPVLPLTSAAVTNLSVTGTSDTTSNNTATVISTAPPASSAVTSPSLSPSPSASASTSEASSASETSTTQTTSTPLSSPLGTSQVMVTASGLQTAAAAALQGAAQLPANASLAAVAAAAGLNPSLMAPSQFAAGGALPSLNPGTLSGALSPALMSNSTLATIQALASGGSLPITSLDATGNLVFANAGGAPNIVTAPLFLNPQNLSLLTSNPVSLVSAAAASAGNSAPVASLHATSTSAESIQNSLFTVASASGAASTTTTASKAQ
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
107.47
Interspecies Antigen Sequence
Mouse (89); Rat (95)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — POU2F1
Entrez GeneID
5451GeneBank Accession#
BC001664Protein Accession#
AAH01664Gene Name
POU2F1
Gene Alias
OCT1, OTF1
Gene Description
POU class 2 homeobox 1
Omim ID
164175Gene Ontology
HyperlinkGene Summary
class 2
Other Designations
OTTHUMP00000032348|Octamer-binding transcription factor-1|POU domain, class 2, transcription factor 1
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Interactome
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Disease
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Publication Reference
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Methyl CpG Level at Distal Part of Heat-Shock Protein Promoter HSP70 Exhibits Epigenetic Memory for Heat Stress by Modulating Recruitment of POU2F1-Associated Nucleosome Remodeling Deacetylase (NuRD) Complex.
Kisliouk T, Cramer T, Meiri N.
Journal of Neurochemistry 2017 May; 141(3):358.
Application:Func, Probes.
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Allele-specific transcription of the asthma-associated PHD finger protein 11 gene (PHF11) modulated by octamer-binding transcription factor 1 (Oct-1).
Holt RJ, Zhang Y, Binia A, Dixon AL, Vandiedonck C, Cookson WO, Knight JC, Moffatt MF.
The Journal of Allergy and Clinical Immunology 2011 Apr; 127(4):1054.
Application:Func, Human, CCL-159, Daudi cells.
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Methyl CpG Level at Distal Part of Heat-Shock Protein Promoter HSP70 Exhibits Epigenetic Memory for Heat Stress by Modulating Recruitment of POU2F1-Associated Nucleosome Remodeling Deacetylase (NuRD) Complex.
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