PIK3R1 monoclonal antibody (M01), clone 3A10
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More Files
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Western Blot (Cell lysate)
PIK3R1 monoclonal antibody (M01), clone 3A10. Western Blot analysis of PIK3R1 expression in NIH/3T3 ( Cat # L018V1 ).
Western Blot (Cell lysate)
PIK3R1 monoclonal antibody (M01), clone 3A10. Western Blot analysis of PIK3R1 expression in Raw 264.7 ( Cat # L024V1 ).
Western Blot (Cell lysate)
PIK3R1 monoclonal antibody (M01), clone 3A10. Western Blot analysis of PIK3R1 expression in PC-12 ( Cat # L012V1 ).
Western Blot (Cell lysate)
PIK3R1 monoclonal antibody (M01), clone 3A10 Western Blot analysis of PIK3R1 expression in HeLa ( Cat # L013V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of PIK3R1 expression in transfected 293T cell line by PIK3R1 monoclonal antibody (M01), clone 3A10.
Lane 1: PIK3R1 transfected lysate (Predicted MW: 50 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of PIK3R1 transfected lysate using anti-PIK3R1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PIK3R1 monoclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PIK3R1 is approximately 0.1ng/ml as a capture antibody.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between EGFR and PIK3R1. HeLa cells were stained with anti-EGFR rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between FLT1 and PIK3R1. Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between CRKL and PIK3R1. Mahlavu cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blot detection against Immunogen (75.68 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant PIK3R1.
Immunogen
PIK3R1 (AAH30815, 1 a.a. ~ 454 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MYNTVWNMEDLDLEYAKTDINCGTDLMFYIEMDPPALPPKPPKPTTVANNGMNNNMSLQDAEWYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFSSVVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLHKYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCQTQERYSKEYIEKFKREGNEKEIQRIMHNYDKLKSRISEIIDSRRRLEEDLKKQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQHTSLVQHNDSLNVTLAYPVYAQQRR
Host
Mouse
Reactivity
Human, Mouse, Rat
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (75.68 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
PIK3R1 monoclonal antibody (M01), clone 3A10. Western Blot analysis of PIK3R1 expression in NIH/3T3 ( Cat # L018V1 ).Western Blot (Cell lysate)
PIK3R1 monoclonal antibody (M01), clone 3A10. Western Blot analysis of PIK3R1 expression in Raw 264.7 ( Cat # L024V1 ).Western Blot (Cell lysate)
PIK3R1 monoclonal antibody (M01), clone 3A10. Western Blot analysis of PIK3R1 expression in PC-12 ( Cat # L012V1 ).Western Blot (Cell lysate)
PIK3R1 monoclonal antibody (M01), clone 3A10 Western Blot analysis of PIK3R1 expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of PIK3R1 expression in transfected 293T cell line by PIK3R1 monoclonal antibody (M01), clone 3A10.
Lane 1: PIK3R1 transfected lysate (Predicted MW: 50 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of PIK3R1 transfected lysate using anti-PIK3R1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PIK3R1 monoclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PIK3R1 is approximately 0.1ng/ml as a capture antibody.ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between EGFR and PIK3R1. HeLa cells were stained with anti-EGFR rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between FLT1 and PIK3R1. Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between CRKL and PIK3R1. Mahlavu cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — PIK3R1
Entrez GeneID
5295GeneBank Accession#
BC030815Protein Accession#
AAH30815Gene Name
PIK3R1
Gene Alias
GRB1, p85, p85-ALPHA
Gene Description
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
Omim ID
171833Gene Ontology
HyperlinkGene Summary
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)|phosphatidylinositol 3-kinase, regulatory, 1|phosphatidylinositol 3-kinase-associated p-85 alpha|phosphoinositide-3-kinase, regulatory subunit 1 (p85 alpha)|phosphoinositide-3-ki
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Interactome
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Pathway
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Disease
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Publication Reference
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An analysis of protein-protein interactions in cross-talk pathways reveals CRKL as a novel prognostic marker in hepatocellular carcinoma.
Liu CH, Chen TC, Chau GY, Jan YH, Chen CH, Hsu CN, Lin KT, Juang YL, Lu PJ, Cheng HC, Chen MH, Chang CF, Ting YS, Kao CY, Hsiao M, Huang CY.
Molecular & Cellular Proteomics 2013 May; 12(5):1335.
Application：Profiling, Human, Huh7 cells, Mahlavu cells.
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An analysis of protein-protein interactions in cross-talk pathways reveals CRKL as a novel prognostic marker in hepatocellular carcinoma.
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