PDK4 (Human) Recombinant Protein (Q01)
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More Files
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Specification
Product Description
Human PDK4 partial ORF ( AAH40239, 181 a.a. - 280 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
SDSQTGNPSHIGSIDPNCDVVAVVQDAFGCSRMLCDQYYLSSPELKLTQANGKFPDQPIHIVYVPSHLHHMLFELFKNAMRATVEHQENQPSLTPIEVIV
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
36.63
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — PDK4
Entrez GeneID
5166GeneBank Accession#
BC040239Protein Accession#
AAH40239Gene Name
PDK4
Gene Alias
FLJ40832
Gene Description
pyruvate dehydrogenase kinase, isozyme 4
Omim ID
602527Gene Ontology
HyperlinkGene Summary
This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. [provided by RefSeq
Other Designations
pyruvate dehydrogenase kinase 4|pyruvate dehydrogenase kinase, isoenzyme 4
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