PCBP2 monoclonal antibody (M07), clone 5F12
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More Files
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Western Blot (Cell lysate)
PCBP2 monoclonal antibody (M07), clone 5F12 Western Blot analysis of PCBP2 expression in K-562 ( Cat # L009V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of PCBP2 expression in transfected 293T cell line by PCBP2 monoclonal antibody (M07), clone 5F12.
Lane 1: PCBP2 transfected lysate(38.7 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PCBP2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PCBP2 is approximately 0.1ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of PCBP2 over-expressed 293 cell line, cotransfected with PCBP2 Validated Chimera RNAi ( Cat # H00005094-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PCBP2 monoclonal antibody (M07), clone 5F12 (Cat # H00005094-M07 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunofluorescence
Immunofluorescence of monoclonal antibody to PCBP2 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (65.56 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant PCBP2.
Immunogen
PCBP2 (AAH01155, 1 a.a. ~ 362 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MDTGVIEGGLNVTLTIRLLMHGKEVGSIIGKKGESVKKMREESGARINISEGNCPERIITLAGPTNAIFKAFAMIIDKLEEDISSSMTNSTAASRPPVTLRLVVPASQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGIPQSIIECVKQICVVMLESPPKGVTIPYRPKPSSSPVIFAGGQDRYSTGSDSASFPHTTPSMCLNPDLEGPPLEAYTIQGQYAIPQPDLTKLHQLAMQQSHFPMTHGNTGFSGIESSSPEVKGYWAGLDASAQTTSHELTIPNDLIGCIIGRQGAKINEIRQMSGAQIKIANPVEGSTDRQVTITGSAASISLAQYLINVRLSSETGGMGSS
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (100); Rat (99)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (65.56 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
PCBP2 monoclonal antibody (M07), clone 5F12 Western Blot analysis of PCBP2 expression in K-562 ( Cat # L009V1 ).Western Blot (Transfected lysate)
Western Blot analysis of PCBP2 expression in transfected 293T cell line by PCBP2 monoclonal antibody (M07), clone 5F12.
Lane 1: PCBP2 transfected lysate(38.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PCBP2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PCBP2 is approximately 0.1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of PCBP2 over-expressed 293 cell line, cotransfected with PCBP2 Validated Chimera RNAi ( Cat # H00005094-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PCBP2 monoclonal antibody (M07), clone 5F12 (Cat # H00005094-M07 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to PCBP2 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — PCBP2
Entrez GeneID
5094GeneBank Accession#
BC001155Protein Accession#
AAH01155Gene Name
PCBP2
Gene Alias
HNRPE2, MGC110998, hnRNP-E2
Gene Description
poly(rC) binding protein 2
Omim ID
601210Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
alpha-CP2|heterogenous nuclear ribonucleoprotein E2
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Interactome
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Publication Reference
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Binding of microRNA-122 to the hepatitis C virus 5' untranslated region modifies interactions with poly(C) binding protein 2 and the NS5B viral polymerase.
Seth Scott, You Li, Oya Bermek, Jack D Griffith, Stanley M Lemon, Kyung H Choi.
Nucleic Acids Research 2023 Dec; 51(22):12397.
Application：WB, N/A, Biotinylated HCV RNA.
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Mutated FUS in familial amyotrophic lateral sclerosis involves multiple hnRNPs in the formation of neuronal cytoplasmic inclusions.
Hiroyuki Honda, Motoi Yoshimura, Hajime Arahata, Kaoru Yagita, Shoko Sadashima, Hideomi Hamasaki, Masahiro Shijo, Sachiko Koyama, Hideko Noguchi, Naokazu Sasagasako.
Journal of Neuropathology and Eexperimental Neurology 2023 Feb; 82(3):231.
Application：IF, IHC-P, Human, Human brains.
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PCBP2 Is Downregulated in Degenerating Neurons and Rarely Observed in TDP-43-Positive Inclusions in Sporadic Amyotrophic Lateral Sclerosis.
Motoi Yoshimura, Hiroyuki Honda, Naokazu Sasagasako, Shinichiro Mori, Hideomi Hamasaki, Satoshi O Suzuki, Takashi Ishii, Toshiharu Ninomiya, Jun-Ichi Kira, Toru Iwaki.
Journal of Neuropathology and Eexperimental Neurology 2021 Feb; 80(3):220.
Application：IF, IHC-P, WB-Tr, Human, HeLa cells, Human amyotrophic lateral sclerosis, Human spinal anterior horn cells, Human spinal cord.
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A Network of RNA-binding Proteins Controls Translation Efficiency to Activate Anaerobic Metabolism.
J J David Ho, Nathan C Balukoff, Phaedra R Theodoridis, Miling Wang, Jonathan R Krieger, Jonathan H Schatz, Stephen Lee.
Nature Communications 2020 May; 11(1):2677.
Application：WB-Ce, WB-Tr, Human, U87 MG cells.
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Flavivirus Infection Uncouples Translation Suppression from Cellular Stress Responses.
Roth H, Magg V, Uch F, Mutz P, Klein P, Haneke K, Lohmann V, Bartenschlager R, Fackler OT, Locker N, Stoecklin G, Ruggieri A.
Mbio 2017 Jan; 8(1):e02150.
Application：IF, Human, Huh7 cells.
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Iron Export through the Transporter Ferroportin 1 Is Modulated by the Iron Chaperone PCBP2.
Yanatori I, Richardson DR, Imada K, Kishi F.
The Journal of Biological Chemistry 2016 Aug; 291(33):17303.
Application：WB-Tr, Human, HEp-2 cells.
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Poly(rC)-Binding Protein 2 Regulates Hippo Signaling To Control Growth in Breast Epithelial Cells.
Li F, Bullough KZ, Vashisht AA, Wohlschlegel JA, Philpott CC.
Molecular and Cellular Biology 2016 May; 36(16):2121.
Application：IP-WB, WB-Ce, WB-Tr, Human, MCF-7, MCF-10A, MDA-MB-231 cells.
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Iron Toxicity in the Retina Requires Alu RNA and the NLRP3 Inflammasome.
Bradley D Gelfand, Charles B Wright, Younghee Kim, Tetsuhiro Yasuma, Reo Yasuma, Shengjian Li, Benjamin J Fowler, Ana Bastos-Carvalho, Nagaraj Kerur, Annette Uittenbogaard, Youn Seon Han, Dingyuan Lou, Mark E Kleinman, W Hayes McDonald, Gabriel Núñez, Philippe Georgel, Joshua L Dunaief, Jayakrishna Ambati.
Cell Reports 2015 Jun; 11(11):1686.
Application：Pull-Down, Human, ARPE-19 cells.
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hnRNP L and NF90 interact with hepatitis C virus 5' terminal untranslated RNA and promote efficient replication.
Li Y, Masaki T, Shimakami T, Lemon SM.
Journal of Virology 2014 Jul; 88(13):7199.
Application：WB-Tr, Human, Huh7.5 cells.
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Selenite targets eIF4E-binding protein-1 to inhibit translation initiation and induce the assembly of non-canonical stress granules.
Fujimura K, Sasaki AT, Anderson P.
Nucleic Acids Research 2012 Sep; 40(16):8099.
Application：IF, Human, U2OS cells.
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Poly(C)-binding protein 2 Interacts with sequences Required for Viral Replication in HCV 5' UTR and Directs HCV RNA Replication through Circularizing the Viral Genome.
Wang L, Jeng KS, Lai MM.
J Virol 2011 Jun; 85:7954.
Application：IF, WB-Ce, WB-Tr, Human, Huh-7 cells.
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Identification of RNA Helicase A (RHA) as a New Host Factor in the Replication Cycle of Foot-and-Mouth Disease Virus.
Lawrence P, Rieder E.
Journal of Virology 2009 Nov; 83(21):11356.
Application：IF, IP, Human, LFBK cells.
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Selective localization of PCBP2 to cytoplasmic processing bodies.
Fujimura K, Katahira J, Kano F, Yoneda Y, Murata M.
Biochimica et Biophysica Acta 2009 May; 1793(5):878.
Application：IF, Human, HeLa cells.
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Rapid Changes of mRNA-binding Protein Levels following Glucose and 3-Isobutyl-1-methylxanthine Stimulation of Insulinoma INS-1 Cells.
Sus C, Czupalla C, Winter C, Pursche T, Knoch KP, Schroeder M, Hoflack B, Solimena M.
Molecular & Cellular Proteomics 2008 Oct; 8(3):393.
Application：WB, Rat, INS-1 cells.
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A link between SIN1 (MAPKAP1) and poly(rC) binding protein 2 (PCBP2) in counteracting environmental stress.
Ghosh D, Srivastava GP, Xu D, Schulz LC, Roberts RM.
PNAS 2008 Aug; 105(33):11673.
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Identification of PCBP2, a facilitator of IRES-mediated translation, as a novel constituent of stress granules and processing bodies.
Fujimura K, Kano F, Murata M.
RNA 2008 Jan; 14(3):425.
Application：IF, WB, Human, HeLa cells.
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Binding of microRNA-122 to the hepatitis C virus 5' untranslated region modifies interactions with poly(C) binding protein 2 and the NS5B viral polymerase.
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