MCM7 monoclonal antibody (M01), clone 6C2
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant MCM7.
Immunogen
MCM7 (AAH09398, 1 a.a. ~ 389 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MALKDYALEKEKVKKFLQEFYQDDELGKKQFKYGNQLVRLAHREQVALYVDLDDVAEDDPELVDSICENARRYAKLFADAVQELLPQYKEREVVNKDVLDVYIEHRLMMEQRSRDPGMVRSPQNQYPAELMRRFELYFQGPSSSKPRVIREVRADSVGKLVTVRGIVTRVSEVKPKMVVATYTCDQCGAETYQPIQSPTFMPLIMCPSQECQTNRSGGRLYLQTRGSRFIKFQEMKMQEHSDQVPVGNIPRSITVLVEGENTRIAQPGDHVSVTGIFLPILRTGFRQVVQGLLSETYLEAHRIVKMNKSEDDESGAGELTREELRQIADVIFATVRELVSGGRSVRFSEAEQRCVSRGFTPAQFQAALDEYEELNVWQVNASRTRITFV
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (68.53 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
MCM7 monoclonal antibody (M01), clone 6C2 Western Blot analysis of MCM7 expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to MCM7 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MCM7 is approximately 0.1ng/ml as a capture antibody.ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between CDK7 and MCM7. HeLa cells were stained with anti-CDK7 rabbit purified polyclonal 1:1200 and anti-MCM7 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between CDC6 and MCM7. Huh7 cells were stained with anti-CDC6 rabbit purified polyclonal 1:600 and anti-MCM7 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Immunofluorescence
Immunofluorescence of monoclonal antibody to MCM7 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — MCM7
Entrez GeneID
4176GeneBank Accession#
BC009398Protein Accession#
AAH09398Gene Name
MCM7
Gene Alias
CDABP0042, CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS-146
Gene Description
minichromosome maintenance complex component 7
Omim ID
600592Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
DNA replication licensing factor MCM7|MCM7 minichromosome maintenance deficient 7|homolog of S. cerevisiae Cdc47|minichromosome maintenance deficient 7
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Interactome
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Pathway
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Disease
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Publication Reference
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Etiological Role of Human Papillomavirus Infection in the Development of Penile Cancer.
Sakamoto J, Shigehara K, Nakashima K, Kawaguchi S, Nakashima T, Shimamura M, Yasuda M, Kato T, Hasegawa T, Kobori Y, Okada H, Deguchi T, Izumi K, Kadono Y, Mizokami A.
International Journal of Infectious Diseases 2019 Jan; 78:148.
Application:IHC-P, Human, Human penile cancer.
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Etiological role of human papillomavirus infection for inverted papilloma of the bladder.
Shigehara K, Sasagawa T, Doorbar J, Kawaguchi S, Kobori Y, Nakashima T, Shimamura M, Maeda Y, Miyagi T, Kitagawa Y, Kadono Y, Konaka H, Mizokami A, Koh E, Namiki M.
Journal of Medical Virology 2011 Feb; 83(2):277.
Application:IHC, Human, Papilloma.
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Etiologic role of human papillomavirus infection in bladder carcinoma.
Shigehara K, Sasagawa T, Kawaguchi S, Nakashima T, Shimamura M, Maeda Y, Konaka H, Mizokami A, Koh E, Namiki M.
Cancer 2011 May; 117(10):2067.
Application:IHC-P, Human, Bladder Carcinoma.
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Minichromosome maintenance proteins 2, 3 and 7 in medulloblastoma: overexpression and involvement in regulation of cell migration and invasion.
Lau KM, Chan QK, Pang JC, Li KK, Yeung WW, Chung NY, Lui PC, Tam YS, Li HM, Zhou L, Wang Y, Mao Y, Ng HK.
Oncogene 2010 Oct; 29(40):5475.
Application:IHC-P, WB-Tr, Human, DAOY cells, Human medulloblastoma.
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Validation of different replication markers for the detection of beta-cell proliferation in human pancreatic tissue.
Kohler CU, Kreuter A, Rozynkowski MC, Rahmel T, Uhl W, Tannapfel A, Schmidt WE, Meier JJ.
Regulatory Peptides 2010 Jun; 162(1-3):115.
Application:IF, IHC-P, Human, Human pancreatic tissue sections.
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Etiological Role of Human Papillomavirus Infection in the Development of Penile Cancer.
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