LIPA 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
LIPA transfected lysate.
Western Blot
Lane 1: LIPA transfected lysate ( 44 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-LIPA full-length
Host
Human
Theoretical MW (kDa)
44
Interspecies Antigen Sequence
Mouse (74); Rat (76)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-LIPA antibody (H00003988-B01) by Western Blots.
SDS-PAGE Gel
LIPA transfected lysate.
Western Blot
Lane 1: LIPA transfected lysate ( 44 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — LIPA
Entrez GeneID
3988GeneBank Accession#
BC012287.1Protein Accession#
AAH12287.1Gene Name
LIPA
Gene Alias
CESD, LAL
Gene Description
lipase A, lysosomal acid, cholesterol esterase
Omim ID
278000Gene Ontology
HyperlinkGene Summary
This gene encodes lipase A, the lysosomal acid lipase (also known as cholesterol ester hydrolase). This enzyme functions in the lysosome to catalyze the hydrolysis of cholesteryl esters and triglycerides. Mutations in this gene can result in Wolman disease and cholesteryl ester storage disease. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000020066|OTTHUMP00000020068|OTTHUMP00000059643|cholesterol ester hydrolase|lipase A|lysosomal acid lipase|sterol esterase
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Interactome
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Pathway
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Disease
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