KRT19 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: KRT19 transfected lysate ( 44 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
KRT19 transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-KRT19 full-length
Host
Human
Theoretical MW (kDa)
44.11
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-KRT19 antibody (H00003880-B01) by Western Blots.
Western Blot
Lane 1: KRT19 transfected lysate ( 44 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
KRT19 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — KRT19
Entrez GeneID
3880GeneBank Accession#
BC007628Protein Accession#
AAH07628Gene Name
KRT19
Gene Alias
CK19, K19, K1CS, MGC15366
Gene Description
keratin 19
Omim ID
148020Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq
Other Designations
40-kDa keratin intermediate filament|cytokeratin 19|keratin, type I cytoskeletal 19|keratin, type I, 40-kd
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