KRT18 monoclonal antibody (M01), clone 2F8
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More Files
- More Functions
Western Blot (Cell lysate)
KRT18 monoclonal antibody (M01), clone 2F8 Western Blot analysis of KRT18 expression in HeLa ( Cat # L013V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of KRT18 expression in transfected 293T cell line by KRT18 monoclonal antibody (M01), clone 2F8.
Lane 1: KRT18 transfected lysate(48.1 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to KRT18 on formalin-fixed paraffin-embedded human liver tissue. [antibody concentration 3 ug/ml]
Immunoprecipitation
Immunoprecipitation of KRT18 transfected lysate using anti-KRT18 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with KRT18 MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged KRT18 is approximately 0.1ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to KRT18 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (47.41 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant KRT18.
Immunogen
KRT18 (AAH00180, 1 a.a. ~ 430 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSFTTRSTFSTNYRSLGSVQAPSYGARSVSSAASVYAGAGGSGSRISVSRSTSFRGGMGSGGLATGIAGGLAGMGGIQNEKETMQSLNDRPASYLDRVRSLETENRRLESKIREHLEKKGPQVRDWSHYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADDFRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVKGLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVSETNDTKVLRY
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (85); Rat (83)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (47.41 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
KRT18 monoclonal antibody (M01), clone 2F8 Western Blot analysis of KRT18 expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of KRT18 expression in transfected 293T cell line by KRT18 monoclonal antibody (M01), clone 2F8.
Lane 1: KRT18 transfected lysate(48.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to KRT18 on formalin-fixed paraffin-embedded human liver tissue. [antibody concentration 3 ug/ml]Immunoprecipitation
Immunoprecipitation of KRT18 transfected lysate using anti-KRT18 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with KRT18 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged KRT18 is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to KRT18 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — KRT18
Entrez GeneID
3875GeneBank Accession#
BC000180Protein Accession#
AAH00180Gene Name
KRT18
Gene Alias
CYK18, K18
Gene Description
keratin 18
Gene Ontology
HyperlinkGene Summary
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
cell proliferation-inducing protein 46|cytokeratin 18
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Interactome
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Pathway
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Disease
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