IL1B monoclonal antibody (M01), clone 2A8
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More Files
- More Functions
Western Blot (Tissue lysate)
IL1B monoclonal antibody (M01), clone 2A8. Western Blot analysis of IL1B expression in human liver.
Immunoprecipitation
Immunoprecipitation of IL1B transfected lysate using anti-IL1B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IL1B MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged IL1B is approximately 10ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to IL1B on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (36.63 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant IL1B.
Immunogen
IL1B (AAH08678, 170 a.a. ~ 269 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
DKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS
Host
Mouse
Reactivity
Human
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
IL1B monoclonal antibody (M01), clone 2A8. Western Blot analysis of IL1B expression in human liver.Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of IL1B transfected lysate using anti-IL1B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IL1B MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged IL1B is approximately 10ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to IL1B on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — IL1B
Entrez GeneID
3553GeneBank Accession#
BC008678Protein Accession#
AAH08678Gene Name
IL1B
Gene Alias
IL-1, IL1-BETA, IL1F2
Gene Description
interleukin 1, beta
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq
Other Designations
catabolin|preinterleukin 1 beta|pro-interleukin-1-beta
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Interactome
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Pathway
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Disease
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Publication Reference
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Upregulation of MMP-13 via Runx2 in the stromal cell of Giant Cell Tumor.
Mak IW, Cowan RW, Popovic S, Colterjohn N, Singh G, Ghert M.
Bone 2009 Aug; 45(2):377.
Application：IHC-P, Human, Human giant cell tumors.
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Upregulation of MMP-13 via Runx2 in the stromal cell of Giant Cell Tumor.
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