HSPA1B monoclonal antibody (M02), clone 3B7
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More Files
- More Functions
Western Blot (Cell lysate)
HSPA1B monoclonal antibody (M02), clone 3B7. Western Blot analysis of HSPA1B expression in Raw 264.7 ( Cat # L024V1 ).
Western Blot (Cell lysate)
HSPA1B monoclonal antibody (M02), clone 3B7. Western Blot analysis of HSPA1B expression in PC-12 ( Cat # L012V1 ).
Western Blot (Cell lysate)
HSPA1B monoclonal antibody (M02), clone 3B7 Western Blot analysis of HSPA1B expression in IMR-32 ( Cat # L008V1 ).
Western Blot (Cell lysate)
HSPA1B monoclonal antibody (M02), clone 3B7. Western Blot analysis of HSPA1B expression in NIH/3T3 ( Cat # L018V1 ).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to HSPA1B on formalin-fixed paraffin-embedded human testis. [antibody concentration 6 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HSPA1B is approximately 0.03ng/ml as a capture antibody.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between TP53 and HSPA1B. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-HSPA1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunofluorescence
Immunofluorescence of monoclonal antibody to HSPA1B on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (34.65 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant HSPA1B.
Immunogen
HSPA1B (NP_005336, 531 a.a. ~ 611 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
VQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLY
Host
Mouse
Reactivity
Human, Mouse, Rat
Interspecies Antigen Sequence
Mouse (88); Rat (93)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (34.65 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
HSPA1B monoclonal antibody (M02), clone 3B7. Western Blot analysis of HSPA1B expression in Raw 264.7 ( Cat # L024V1 ).Western Blot (Cell lysate)
HSPA1B monoclonal antibody (M02), clone 3B7. Western Blot analysis of HSPA1B expression in PC-12 ( Cat # L012V1 ).Western Blot (Cell lysate)
HSPA1B monoclonal antibody (M02), clone 3B7 Western Blot analysis of HSPA1B expression in IMR-32 ( Cat # L008V1 ).Western Blot (Cell lysate)
HSPA1B monoclonal antibody (M02), clone 3B7. Western Blot analysis of HSPA1B expression in NIH/3T3 ( Cat # L018V1 ).Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to HSPA1B on formalin-fixed paraffin-embedded human testis. [antibody concentration 6 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HSPA1B is approximately 0.03ng/ml as a capture antibody.ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between TP53 and HSPA1B. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-HSPA1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Immunofluorescence
Immunofluorescence of monoclonal antibody to HSPA1B on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — HSPA1B
Entrez GeneID
3304GeneBank Accession#
NM_005345Protein Accession#
NP_005336Gene Name
HSPA1B
Gene Alias
FLJ54328, HSP70-1B, HSP70-2, HSPA1A
Gene Description
heat shock 70kDa protein 1B
Omim ID
603012Gene Ontology
HyperlinkGene Summary
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq
Other Designations
OTTHUMP00000029288|heat shock 70kD protein 1B|heat shock 70kDa protein 1A
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Interactome
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Pathway
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Disease
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Publication Reference
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The study of protein biomarkers to understand the biochemical processes underlying beef color development in young bulls.
Gagaoua M, Terlouw EMC, Picard B.
Meat Science 2017 Jul; 134:18.
Application：Dot, Bovine, Bovine muscle.
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Inverse relationships between biomarkers and beef tenderness according to contractile and metabolic properties of the muscle.
Picard B, Gagaoua M, Micol D, Cassar-Malek I, Hocquette JF, Terlouw CE.
Journal of Agricultural and Food Chemistry 2014 Oct; 62(40):9808.
Application：Dot, Bovine, Bovine muscle.
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Functional analysis of beef tenderness.
Guillemin N, Bonnet M, Jurie C, Picard B.
Journal of Proteomics 2011 Dec; 75(2):352.
Application：WB-Ti, Bovine, Bovine tenderness.
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The study of protein biomarkers to understand the biochemical processes underlying beef color development in young bulls.
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