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HRAS 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00003265-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-HRAS full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 21.3
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-HRAS antibody (H00003265-B02) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00003265-T01
    HRAS transfected lysate.
    Western Blot
    QC Testing of H00003265-T01
    Lane 1: HRAS transfected lysate ( 18.81 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 3265
  • Gene Name:
  • HRAS
  • Gene Alias:
  • C-BAS/HAS,C-H-RAS,C-HA-RAS1,CTLO,H-RASIDX,HAMSV,HRAS1,K-RAS,N-RAS,RASH1
  • Gene Description:
  • v-Ha-ras Harvey rat sarcoma viral oncogene homolog
  • Gene Summary:
  • This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq
  • Other Designations:
  • GTP- and GDP-binding peptide B,GTPase HRas,Ha-Ras1 proto-oncoprotein,OTTHUMP00000162769,OTTHUMP00000166053,OTTHUMP00000166055,Ras family small GTP binding protein H-Ras,c-has/bas p21 protein,c-ras-Ki-2 activated oncogene,p19 H-RasIDX protein,transformatio
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