HRAS 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-HRAS full-length
Host
Human
Theoretical MW (kDa)
21.3
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-HRAS antibody (H00003265-B02) by Western Blots.
SDS-PAGE Gel
HRAS transfected lysate.
Western Blot
Lane 1: HRAS transfected lysate ( 18.81 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — HRAS
Entrez GeneID
3265GeneBank Accession#
NM_005343Protein Accession#
AAH95471.1Gene Name
HRAS
Gene Alias
C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, K-RAS, N-RAS, RASH1
Gene Description
v-Ha-ras Harvey rat sarcoma viral oncogene homolog
Gene Ontology
HyperlinkGene Summary
This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq
Other Designations
GTP- and GDP-binding peptide B|GTPase HRas|Ha-Ras1 proto-oncoprotein|OTTHUMP00000162769|OTTHUMP00000166053|OTTHUMP00000166055|Ras family small GTP binding protein H-Ras|c-has/bas p21 protein|c-ras-Ki-2 activated oncogene|p19 H-RasIDX protein|transformatio
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Interactome
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Pathway
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Disease
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