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HLA-E 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00003133-T02
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-HLA-E full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 40.2
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-HLA-E antibody (H00003133-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00003133-T02
    HLA-E transfected lysate.
    Western Blot
    QC Testing of H00003133-T02
    Lane 1: HLA-E transfected lysate ( 40.20 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 3133
  • Gene Name:
  • HLA-E
  • Gene Alias:
  • DKFZp686P19218,EA1.2,EA2.1,HLA-6.2,MHC,QA1
  • Gene Description:
  • major histocompatibility complex, class I, E
  • Gene Summary:
  • HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq
  • Other Designations:
  • HLA class I histocompatibility antigen, E alpha chain,MHC HLA-E alpha-1,MHC HLA-E alpha-2.1,MHC class I antigen,OTTHUMP00000029214,lymphocyte antigen
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