Transient overexpression cell lysate was tested with Anti-HFE antibody (H00003077-B01) by Western Blots. SDS-PAGE Gel HFE transfected lysate. Western Blot Lane 1: HFE transfected lysate ( 40.1 KDa) Lane 2: Non-transfected lysate.
Storage Buffer:
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined. [provided by RefSeq
Other Designations:
MHC class I-like protein HFE,hemochromatosis protein,hereditary hemochromatosis protein HLA-H