HARS MaxPab mouse polyclonal antibody (B01)
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More Files
- More Functions
Western Blot (Tissue lysate)
HARS MaxPab polyclonal antibody. Western Blot analysis of HARS expression in human liver.
Western Blot (Cell lysate)
HARS MaxPab polyclonal antibody. Western Blot analysis of HARS expression in HeLa.
Western Blot (Transfected lysate)
Western Blot analysis of HARS expression in transfected 293T cell line (H00003035-T01) by HARS MaxPab polyclonal antibody.
Lane 1: HARS transfected lysate(55.99 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to HARS on HeLa cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human HARS protein.
Immunogen
HARS (NP_002100.2, 1 a.a. ~ 509 a.a) full-length human protein.
Sequence
MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARGLDYYTGVIYEAVLLQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKIRTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQPLCIC
Host
Mouse
Reactivity
Human
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
No additive
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Note
For IHC and IF applications, antibody purification with Protein A will be needed prior to use.
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Applications
Western Blot (Tissue lysate)
HARS MaxPab polyclonal antibody. Western Blot analysis of HARS expression in human liver.Western Blot (Cell lysate)
HARS MaxPab polyclonal antibody. Western Blot analysis of HARS expression in HeLa.Western Blot (Transfected lysate)
Western Blot analysis of HARS expression in transfected 293T cell line (H00003035-T01) by HARS MaxPab polyclonal antibody.
Lane 1: HARS transfected lysate(55.99 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to HARS on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — HARS
Entrez GeneID
3035GeneBank Accession#
NM_002109.3Protein Accession#
NP_002100.2Gene Name
HARS
Gene Alias
FLJ20491, HRS
Gene Description
histidyl-tRNA synthetase
Omim ID
142810Gene Ontology
HyperlinkGene Summary
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. [provided by RefSeq
Other Designations
HisRS|histidine tRNA ligase 1, cytoplasmic|histidine translase|histidine-tRNA ligase
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Interactome
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Pathway
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