GUCA1A monoclonal antibody (M04), clone 2F7 [Specification] - H00002978-M04

GUCA1A monoclonal antibody (M04), clone 2F7

Catalog # : H00002978-M04

Size :

[ 100 ug ]

Specification

Product Description:		Mouse monoclonal antibody raised against a partial recombinant GUCA1A.

Immunogen:		GUCA1A (NP_000400, 1 a.a. ~ 94 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Immunogen Sequence (without GST):		MGNVMEGKSVEELSSTECHQWYKKFMTECPSGQLTLYEFRQFFGLKNLSP SASQYVEQMFETFDFNKDGYIDFMEYVAALSLVLKGKVEQKLR*

Cross Reactivity:		Human

Isotype:		IgG2b Kappa

Storage Buffer:		In 1x PBS, pH 7.2

Storage Instruction:		Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Quality Control Testing:		Antibody Reactive Against Recombinant Protein. Western Blot detection against Immunogen (36.34 KDa) .

MSDS:		Download

Applications

	Western Blot (Transfected lysate)

	Western Blot analysis of GUCA1A expression in transfected 293T cell line by GUCA1A monoclonal antibody (M04), clone 2F7. Lane 1: GUCA1A transfected lysate(22.9 KDa). Lane 2: Non-transfected lysate.
	Protocol Download

	Western Blot (Recombinant protein)


	Protocol Download

	RNAi Knockdown (Antibody validated)

	Western blot analysis of GUCA1A over-expressed 293 cell line, cotransfected with GUCA1A Validated Chimera RNAi ( Cat # H00002978-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GUCA1A monoclonal antibody (M04), clone 2F7 (Cat # H00002978-M04 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
	Protocol Download

Application Image

Western Blot (Transfected lysate)
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Western Blot (Recombinant protein)

ELISA

RNAi Knockdown (Antibody validated)
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