GTF2H1 monoclonal antibody (M01), clone 1F12-1B5
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant GTF2H1.
Immunogen
GTF2H1 (AAH00365, 1 a.a. ~ 548 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MATSSEEVLLIVKKVRQKKQDGALYLMAERIAWAPEGKDRFTISHMYADIKCQKISPEGKAKIQLQLVLHAGDTTNFHFSNESTAVKERDAVKDLLQQLLPKFKRKANKELEEKNRMLQEDPVLFQLYKDLVVSQVISAEEFWANRLNVNATDSSSTSNHKQDVGISAAFLADVRPQTDGCNGLRYNLTSDIIESIFRTYPAVKMKYAENVPHNMTEKEFWTRFFQSHYFHRDRLNTGSKDLFAECAKIDEKGLKTMVSLGVKNPLLDLTALEDKPLDEGYGISSVPSASNSKSIKENSNAAIIKRFNHHSAMVLAAGLRKQEAQNEQTSEPSNMDGNSGDADCFQPAVKRAKLQESIEYEDLGKNNSVKTIALNLKKSDRYYHGPTPIQSLQYATSQDIINSFQSIRQEMEAYTPKLTQVLSSSAASSTITALSPGGALMQGGTQQAINQMVPNDIQSELKHLYVAVGELLRHFWSCFPVNTPFLEEKVVKMKSNLERFQVTKLCPFQEKIRRQYLSTNLVSHIEEMLQTAYNKLHTWQSRRLMKKT
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97); Rat (91)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (86.02 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
GTF2H1 monoclonal antibody (M01), clone 1F12-1B5. Western Blot analysis of GTF2H1 expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Cell lysate)
GTF2H1 monoclonal antibody (M01), clone 1F12-1B5 Western Blot analysis of GTF2H1 expression in Jurkat ( Cat # L017V1 ).Western Blot (Transfected lysate)
Western Blot analysis of GTF2H1 expression in transfected 293T cell line by GTF2H1 monoclonal antibody (M01), clone 1F12-1B5.
Lane 1: GTF2H1 transfected lysate(62 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to GTF2H1 on formalin-fixed paraffin-embedded human testis tissue.[antibody concentration 5 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GTF2H1 is approximately 1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of GTF2H1 over-expressed 293 cell line, cotransfected with GTF2H1 Validated Chimera RNAi ( Cat # H00002965-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GTF2H1 monoclonal antibody (M01), clone 1F12-1B5 (Cat # H00002965-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to GTF2H1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — GTF2H1
Entrez GeneID
2965GeneBank Accession#
BC000365Protein Accession#
AAH00365Gene Name
GTF2H1
Gene Alias
BTF2, TFB1, TFIIH
Gene Description
general transcription factor IIH, polypeptide 1, 62kDa
Omim ID
189972Gene Ontology
HyperlinkGene Summary
polypeptide 1
Other Designations
general transcription factor IIH, polypeptide 1 (62kD subunit)
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Interactome
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Pathway
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Disease
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Publication Reference
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XPB induces C1D expression to counteract UV-induced apoptosis.
Li G, Liu J, Abu-Asab M, Masabumi S, Maru Y.
Molecular Cancer Research 2010 Jun; 8(6):885.
Application:WB-Ce, Human, HEK 293 cells.
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XPB induces C1D expression to counteract UV-induced apoptosis.
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