GNAS purified MaxPab rabbit polyclonal antibody (D01P)
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against a full-length human GNAS protein.
Immunogen
GNAS (NP_000507.1, 1 a.a. ~ 394 a.a) full-length human protein.
Sequence
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL
Host
Rabbit
Reactivity
Human
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of GNAS expression in transfected 293T cell line (H00002778-T02) by GNAS MaxPab polyclonal antibody.
Lane 1: GNAS transfected lysate(45.70 KDa).
Lane 2: Non-transfected lysate.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between GNAS and ADCY2. HeLa cells were stained with anti-GNAS rabbit purified polyclonal 1:1200 and anti-ADCY2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — GNAS
Entrez GeneID
2778GeneBank Accession#
NM_000516Protein Accession#
NP_000507.1Gene Name
GNAS
Gene Alias
AHO, C20orf45, GNAS1, GPSA, GSA, GSP, MGC33735, NESP, PHP1A, PHP1B, POH, dJ309F20.1.1, dJ806M20.3.3
Gene Description
GNAS complex locus
Gene Ontology
HyperlinkGene Summary
This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contains a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. [provided by RefSeq
Other Designations
OTTHUMP00000031740|OTTHUMP00000031756|OTTHUMP00000196026|OTTHUMP00000196030|adenylate cyclase-stimulating G alpha protein|guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1|guanine nucleotide regulatory protein|neuroe
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Interactome
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Pathway
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Disease
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Publication Reference
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GI protein phosphorylation as marker for scoliosis progression, methods of increasing gipcr signaling subjects.
Alain Moreau, Marie-Yvonne Akoume Ndong.
United States Patent Application Publication 2016 May; [Epub].
Application:PLA-Ce, Det Ab, WB-Tr, Human, PBMCs, Myoblasts.
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GI protein phosphorylation as marker for scoliosis progression, methods of increasing gipcr signaling subjects.
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