GLI1 monoclonal antibody (M01J), clone 1F12
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More Files
- More Functions
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between SUFU and GLI1. HeLa cells were stained with anti-SUFU rabbit purified polyclonal 1:1200 and anti-GLI1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunofluorescence
Immunofluorescence of monoclonal antibody to GLI1 on HeLa cell . [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant GLI1.
This product is belong to Cell Culture Grade Antibody (CX Grade).Immunogen
GLI1 (AAH13000, 401 a.a. ~ 500 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
GPLPRAPSISTVEPKREREGGPIREESRLTVPEGAMKPQPSPGAQSSCSSDHSPAGSAANTDSGVEMTGNAGGSTEDLSSLDEGPCIAGTGLSTLRRLEN
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (88); Rat (89)
Preparation Method
Cell Culture Production
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between SUFU and GLI1. HeLa cells were stained with anti-SUFU rabbit purified polyclonal 1:1200 and anti-GLI1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Immunofluorescence
Immunofluorescence of monoclonal antibody to GLI1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — GLI1
Entrez GeneID
2735GeneBank Accession#
BC013000Protein Accession#
AAH13000Gene Name
GLI1
Gene Alias
GLI
Gene Description
glioma-associated oncogene homolog 1 (zinc finger protein)
Omim ID
165220Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
glioma-associated oncogene homolog (zinc finger protein)|glioma-associated oncogene homolog 1
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Interactome
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Pathway
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Disease
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