GART monoclonal antibody (M01), clone 4D6-1D5
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More Files
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Western Blot (Cell lysate)
GART monoclonal antibody (M01), clone 4D6-1D5 Western Blot analysis of GART expression in Hela S3 NE ( Cat # L013V3 ).
Western Blot (Transfected lysate)
Western Blot analysis of GART expression in transfected 293T cell line by GART monoclonal antibody (M01), clone 4D6-1D5.
Lane 1: GART transfected lysate(46 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to GART on formalin-fixed paraffin-embedded human endometrium tissue. [antibody concentration 2 ug/ml]
Immunoprecipitation
Immunoprecipitation of GART transfected lysate using anti-GART monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GART MaxPab rabbit polyclonal antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to GART on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (73.37 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant GART.
Immunogen
GART (AAH38958.1, 1 a.a. ~ 433 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAARVLIIGSGGREHTLAWKLAQSHHVKQVLVAPGNAGTACSEKISNTAISISDHTALAQFCKEKKIEFVVVGPEAPLAAGIVGNLRSAGVQCFGPTAEAAQLESSKRFAKEFMDRHGIPTAQWKAFTKPEEACSFILSADFPALVVKASGLAAGKGVIVAKSKEEACKAVQEIMQEKAFGAAGETIVIEELLDGEEVSCLCFTDGKTVAPMPPAQDHKRLLEGDGGPNTGGMGAYCPAPQVSNDLLLKIKDTVLQRTVDGMQQEGTPYTGILYAGIMLTKNGPKVLEFNCRFGDPECQVILPLLKSDLYEVIQSTLDGLLCTSLPVWLENHTALTVVMASKGYPGDYTKGVEITGFPEAQALGLEVFHAGTALKNGKVVTHGGRVLAVTAIRENLISALEEAKKGLAAIKFEGAIYRKDVGFRAIAFLQQPR
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (86); Rat (86)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (73.37 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
GART monoclonal antibody (M01), clone 4D6-1D5 Western Blot analysis of GART expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Transfected lysate)
Western Blot analysis of GART expression in transfected 293T cell line by GART monoclonal antibody (M01), clone 4D6-1D5.
Lane 1: GART transfected lysate(46 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to GART on formalin-fixed paraffin-embedded human endometrium tissue. [antibody concentration 2 ug/ml]Immunoprecipitation
Immunoprecipitation of GART transfected lysate using anti-GART monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GART MaxPab rabbit polyclonal antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to GART on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — GART
Entrez GeneID
2618GeneBank Accession#
BC038958Protein Accession#
AAH38958.1Gene Name
GART
Gene Alias
AIRS, GARS, GARTF, MGC47764, PAIS, PGFT, PRGS
Gene Description
phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
Omim ID
138440Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000068280|OTTHUMP00000068281|OTTHUMP00000068282|OTTHUMP00000068337|trifunctional purine biosynthetic protein adenosine-3
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Interactome
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Pathway
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Disease
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Publication Reference
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Proteomic and Bioinformatic Studies for the Characterization of Response to Pemetrexed in Platinum Drug Resistant Ovarian Cancer.
Severi L, Losi L, Fonda S, Taddia L, Gozzi G, Marverti G, Magni F, Chinello C, Stella M, Sheouli J, Braicu EI, Genovese F, Lauriola A, Marraccini C, Gualandi A, D'Arca D, Ferrari S, Costi MP.
Frontiers in Pharmacology 2018 May; 9:454.
Application：WB-Rti, Human, Ovarian carcinoma.
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CRISPR-Cas9 induced mutations along de novo purine synthesis in HeLa cells result in accumulation of individual enzyme substrates and affect purinosome formation.
Baresova V, Krijt M, Skopova V, Souckova O, Kmoch S, Zikanova M.
Molecular Genetics and Metabolism 2016 Aug; 119(3):270.
Application：WB, Human, HeLa cells.
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Mass spectrometric/bioinformatic identification of a protein subset that characterizes the cellular activity of anticancer peptides.
Genovese F, Gualandi A, Taddia L, Marverti G, Pirondi S, Marraccini C, Perco P, Pelà M, Guerrini R, Amoroso MR, Esposito F, Martello A, Ponterini G, D'Arca D, Costi MP.
The Journal of Proteome Research 2014 Nov; 13(11):5250.
Application：WB-Ce, Human, CSD-OC, A2780/CP, IGROV-1 cells.
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Differentially expressed gene profile in the 6-hydroxy-dopamine-induced cell culture model of Parkinson's disease.
Noelker C, Schwake M, Balzer-Geldsetzer M, Bacher M, Popp J, Schlegel J, Eggert K, Oertel WH, Klockgether T, Dodel R.
Neuroscience Letters 2012 Jan; 507(1):10.
Application：IF, WB, Mouse, Human, Primary mesencephalic cells, SH-SY5Y cells.
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No Evidence for Mutations that Deregulate GARS–AIRS–GART Protein Levels in Children with Down Syndrome.
Banerjee D, Ghosh D, Chatterjee A, Sinha S, Nandagopal K.
Indian Journal of Clinical Biochemistry 2012 Jan; 27(1):46.
Application：WB, Human, Serum from patients with Down Syndrome.
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5,10-Methenyltetrahydrofolate Synthetase Activity is Increased in Tumors and Modifies the Efficacy of Antipurine LY309887.
Field MS, Anguera MC, Page R, Stover PJ.
Archives of Biochemistry and Biophysics 2008 Nov; 481(2):145.
Application：WB, Human, SH-SY5Y cells.
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Proteomic and Bioinformatic Studies for the Characterization of Response to Pemetrexed in Platinum Drug Resistant Ovarian Cancer.
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