GABBR1 monoclonal antibody (M01), clone 2D7
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More Files
- More Functions
Western Blot (Cell lysate)
GABBR1 monoclonal antibody (M01), clone 2D7 Western Blot analysis of GABBR1 expression in IMR-32 ( Cat # L008V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of GABBR1 expression in transfected 293T cell line by GABBR1 monoclonal antibody (M01), clone 2D7.
Lane 1: GABBR1 transfected lysate(95 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GABBR1 is approximately 0.3ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of GABBR1 over-expressed 293 cell line, cotransfected with GABBR1 Validated Chimera RNAi ( Cat # H00002550-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GABBR1 monoclonal antibody (M01), clone 2D7 (Cat # H00002550-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunofluorescence
Immunofluorescence of monoclonal antibody to GABBR1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (36.63 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant GABBR1.
Immunogen
GABBR1 (AAH50532, 52 a.a. ~ 151 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
AVYIGALFPMSGGWPGGQACQPAVEMALEDVNSRRDILPDYELKLIHHDSKCDPGQATKYLYELLYNDPIKIILMPGCSSVSTLVAEAARMWNLIVLSYG
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Rat (100)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
GABBR1 monoclonal antibody (M01), clone 2D7 Western Blot analysis of GABBR1 expression in IMR-32 ( Cat # L008V1 ).Western Blot (Transfected lysate)
Western Blot analysis of GABBR1 expression in transfected 293T cell line by GABBR1 monoclonal antibody (M01), clone 2D7.
Lane 1: GABBR1 transfected lysate(95 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GABBR1 is approximately 0.3ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of GABBR1 over-expressed 293 cell line, cotransfected with GABBR1 Validated Chimera RNAi ( Cat # H00002550-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GABBR1 monoclonal antibody (M01), clone 2D7 (Cat # H00002550-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to GABBR1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — GABBR1
Entrez GeneID
2550GeneBank Accession#
BC050532Protein Accession#
AAH50532Gene Name
GABBR1
Gene Alias
FLJ92613, GABAB(1e), GABABR1, GABBR1-3, GPRC3A, dJ271M21.1.1, dJ271M21.1.2, hGB1a
Gene Description
gamma-aminobutyric acid (GABA) B receptor, 1
Omim ID
603540Gene Ontology
HyperlinkGene Summary
Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the mammalian central nervous system. GABA exerts its effects through ionotropic [GABA(A/C)] receptors, to produce fast synaptic inhibition, and metabotropic [GABA(B)] receptors, to produce slow, prolonged inhibitory signals. The GABA(B) receptor consists of a heterodimer of two related 7-transmembrane receptors, GABA(B) receptor 1 and GABA(B) receptor 2. The GABA(B) receptor 1 gene is mapped to chromosome 6p21.3 within the HLA class I region close to the HLA-F gene. Susceptibility loci for multiple sclerosis, epilepsy, and schizophrenia have also been mapped in this region. Alternative splicing of this gene generates multiple transcript variants. [provided by RefSeq
Other Designations
GABA-B receptor|GABAB, subunit 1c|OTTHUMP00000029114|OTTHUMP00000029115|OTTHUMP00000029116|OTTHUMP00000109099|gamma-aminobutyric acid (GABA) B receptor 1|seven transmembrane helix receptor
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Disease
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Publication Reference
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Aversive Learning Increases Release Probability of Olfactory Sensory Neurons.
Janardhan P Bhattarai, Mary Schreck, Andrew H Moberly, Wenqin Luo, Minghong Ma.
Current Biology : CB 2020 Jan; 30(1):31.
Application：IF, Mouse, Mouse brain.
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Limbic encephalitis due to GABAB and AMPA receptor antibodies: a case series.
Dogan Onugoren M, Deuretzbacher D, Haensch CA, Hagedorn HJ, Halve S, Isenmann S, Kramme C, Lohner H, Melzer N, Monotti R, Presslauer S, Schabitz WR, Steffanoni S, Stoeck K, Strittmatter M, Stogbauer F, Trinka E, von Oertzen TJ, Wiendl H, Woermann FG, Bien CG.
Journal of Neurology, Neurosurgery, and Psychiatry 2015 Sep; 86(9):965.
Application：IF, Human, Mouse, Brain, HEK293 cells.
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Conjoint occurrence of GABAB receptor antibodies in Lambert-Eaton myasthenic syndrome with antibodies to the voltage gated calcium channel.
Onugoren MD, Rauschka H, Bien CG.
Journal of Neuroimmunology 2014 Aug; 273(1-2):115.
Application：IF, Human, HEK cells.
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Cortical stimulation causes long-term changes in H-reflexes and spinal motoneuron GABA receptors.
Wang Y, Chen Y, Chen L, Wolpaw JR, Chen XY.
Journal of Neurophysiology 2012 Nov; 108(10):2668.
Application：IHC, Rat, Rat spinal cord.
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Genome-wide association study reveals multiple nasopharyngeal carcinoma-associated loci within the HLA region at chromosome 6p21.3.
Tse KP, Su WH, Chang KP, Tsang NM, Yu CJ, Tang P, See LC, Hsueh C, Yang ML, Hao SP, Li HY, Wang MH, Liao LP, Chen LC, Lin SR, Jorgensen TJ, Chang YS, Shugart YY.
American Journal of Human Genetics 2009 Aug; 85(2):194.
Application：IHC, WB-Ce, Human, BM1, HK1, NP69 cells, Human nasopharyngeal carcinoma.
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Aversive Learning Increases Release Probability of Olfactory Sensory Neurons.
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