FTL monoclonal antibody (M18), clone X3
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More Files
- More Functions
Western Blot (Cell lysate)
FTL monoclonal antibody (M18), clone X3 Western Blot analysis of FTL expression in K-562 ( Cat # L009V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of FTL expression in transfected 293T cell line by FTL monoclonal antibody (M18), clone X3.
Lane 1: FTL transfected lysate(20 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of FTL transfected lysate using anti-FTL monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FTL MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged FTL is approximately 0.03ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of FTL over-expressed 293 cell line, cotransfected with FTL Validated Chimera RNAi ( Cat # H00002512-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FTL monoclonal antibody (M18), clone X3 (Cat # H00002512-M18 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunofluorescence
Immunofluorescence of monoclonal antibody to FTL on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (44.99 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant FTL.
Immunogen
FTL (AAH04245, 1 a.a. ~ 175 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSSQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKHD
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (44.99 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
FTL monoclonal antibody (M18), clone X3 Western Blot analysis of FTL expression in K-562 ( Cat # L009V1 ).Western Blot (Transfected lysate)
Western Blot analysis of FTL expression in transfected 293T cell line by FTL monoclonal antibody (M18), clone X3.
Lane 1: FTL transfected lysate(20 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of FTL transfected lysate using anti-FTL monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FTL MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged FTL is approximately 0.03ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of FTL over-expressed 293 cell line, cotransfected with FTL Validated Chimera RNAi ( Cat # H00002512-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FTL monoclonal antibody (M18), clone X3 (Cat # H00002512-M18 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to FTL on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — FTL
Entrez GeneID
2512GeneBank Accession#
BC004245Protein Accession#
AAH04245Gene Name
FTL
Gene Alias
MGC71996
Gene Description
ferritin, light polypeptide
Gene Ontology
HyperlinkGene Summary
This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq
Other Designations
L apoferritin|ferritin L subunit|ferritin L-chain|ferritin light chain|ferritin light polypeptide-like 3
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Interactome
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Disease
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