FGR 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
FGR transfected lysate.
Western Blot
Lane 1: FGR transfected lysate ( 59.50 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-FGR full-length
Host
Human
Theoretical MW (kDa)
59.5
Interspecies Antigen Sequence
Mouse (84); Rat (85)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-FGR antibody (H00002268-D01P) by Western Blots.
SDS-PAGE Gel
FGR transfected lysate.
Western Blot
Lane 1: FGR transfected lysate ( 59.50 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — FGR
Entrez GeneID
2268GeneBank Accession#
NM_005248.2Protein Accession#
NP_005239.1Gene Name
FGR
Gene Alias
FLJ43153, MGC75096, SRC2, c-fgr, c-src2, p55c-fgr, p58c-fgr
Gene Description
Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog
Omim ID
164940Gene Ontology
HyperlinkGene Summary
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this gene. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000003601|OTTHUMP00000003602|OTTHUMP00000003603|c-fgr protooncogene|c-src-2 proto-oncogene|p55-c-fgr protein|proto-oncogene tyrosine-protein kinase FGR
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Interactome
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Disease
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