PTK2B monoclonal antibody (M01), clone 1F9
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PTK2B.
Immunogen
PTK2B (AAH36651, 682 a.a. ~ 871 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
VYQMEKDIAMEQERNARYRTPKILEPTAFQEPPPKPSRPKYRPPPQTNLLAPKLQFQVPEGLCASSPTLTSPMEYPSPVNSLHTPPLHRHNVFKRHSMREEDFIQPSSREEAQQLWEAEKVKMRQILDKQQKQMVEDYQWLRQEEKSLDPMVYMNDKSPLTPEKEVGYLEFTGPPQKPPRLGAQSIQPTA
Host
Mouse
Reactivity
Human
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (46.53 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
PTK2B monoclonal antibody (M01), clone 1F9 Western Blot analysis of PTK2B expression in K-562 ( Cat # L009V1 ).Western Blot (Transfected lysate)
Western Blot analysis of PTK2B expression in transfected 293T cell line by PTK2B monoclonal antibody (M01), clone 1F9.
Lane 1: PTK2B transfected lysate(115.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of PTK2B transfected lysate using anti-PTK2B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PTK2B MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PTK2B is approximately 0.1ng/ml as a capture antibody.ELISA
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Gene Info — PTK2B
Entrez GeneID
2185GeneBank Accession#
BC036651Protein Accession#
AAH36651Gene Name
PTK2B
Gene Alias
CADTK, CAKB, FADK2, FAK2, FRNK, PKB, PTK, PYK2, RAFTK
Gene Description
PTK2B protein tyrosine kinase 2 beta
Omim ID
601212Gene Ontology
HyperlinkGene Summary
This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
Other Designations
CAK beta|OTTHUMP00000128275|OTTHUMP00000162913|calcium-dependent tyrosine kinase|cell adhesion kinase beta|focal adhesion kinase 2|proline-rich tyrosine kinase 2|protein kinase B|protein tyrosine kinase 2 beta|related adhesion focal tyrosine kinase
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Interactome
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Pathway
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Disease
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Publication Reference
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Key Players of Cisplatin Resistance: Towards a Systems Pharmacology Approach.
Sarin N, Engel F, Rothweiler F, Cinatl J, Michaelis M, Frötschl R, Fröhlich H, Kalayda GV.
International Journal of Molecular Sciences 2018 Mar; 19(3):E767.
Application:WB-Ce, Human, A-549 cells.
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Key Players of Cisplatin Resistance: Towards a Systems Pharmacology Approach.
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