ACSL3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ACSL3 transfected lysate.
Western Blot
Lane 1: ACSL3 transfected lysate ( 79.31 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ACSL3 full-length
Host
Human
Theoretical MW (kDa)
79.31
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ACSL3 antibody (H00002181-B01) by Western Blots.
SDS-PAGE Gel
ACSL3 transfected lysate.
Western Blot
Lane 1: ACSL3 transfected lysate ( 79.31 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ACSL3
Entrez GeneID
2181GeneBank Accession#
NM_004457.3Protein Accession#
NP_004448.2Gene Name
ACSL3
Gene Alias
ACS3, FACL3, PRO2194
Gene Description
acyl-CoA synthetase long-chain family member 3
Omim ID
602371Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000164212|fatty-acid-Coenzyme A ligase, long-chain 3|lignoceroyl-CoA synthase
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