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F8 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00002157-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-F8 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 23.87
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-F8 antibody (H00002157-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00002157-T01
    F8 transfected lysate.
    Western Blot
    QC Testing of H00002157-T01
    Lane 1: F8 transfected lysate ( 23.87 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 2157
  • Protein Accession#:
  • -
  • Gene Name:
  • F8
  • Gene Alias:
  • AHF,DXS1253E,F8B,F8C,FVIII,HEMA
  • Gene Description:
  • coagulation factor VIII, procoagulant component
  • Gene Summary:
  • This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000061446,OTTHUMP00000196174,coagulation factor VIII,coagulation factor VIIIc,factor VIII F8B,procoagulant component
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