F2 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-F2 full-length
Host
Human
Theoretical MW (kDa)
68.53
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-F2 antibody (H00002147-B01) by Western Blots.
Western Blot
Lane 1: F2 transfected lysate ( 70 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
F2 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — F2
Entrez GeneID
2147GeneBank Accession#
NM_000506Protein Accession#
NP_000497Gene Name
F2
Gene Alias
PT
Gene Description
coagulation factor II (thrombin)
Omim ID
176930Gene Ontology
HyperlinkGene Summary
Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. [provided by RefSeq
Other Designations
coagulation factor II|prothrombin B-chain|serine protease
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Interactome
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