F2 monoclonal antibody (M01), clone 1B7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of F2 expression in transfected 293T cell line by F2 monoclonal antibody (M01), clone 1B7.
Lane 1: F2 transfected lysate(70 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of F2 transfected lysate using anti-F2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with F2 MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged F2 is approximately 1ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of F2 over-expressed 293 cell line, cotransfected with F2 Validated Chimera RNAi ( Cat # H00002147-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with F2 monoclonal antibody (M01), clone 1B7 (Cat # H00002147-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between FGA and F2. HeLa cells were stained with anti-FGA rabbit purified polyclonal 1:1200 and anti-F2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant F2.
Immunogen
F2 (AAH51332, 263 a.a. ~ 362 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
RNPDGDEEGAWCYVAGKPGDFGYCDLNYCEEAVEEETGDGLDEDSDRAIEGRTATSEYQTFFNPRTFGSGEADCGLRPLFEKKSLEDKTERELLESYIDG
Host
Mouse
Reactivity
Human
Isotype
IgG
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of F2 expression in transfected 293T cell line by F2 monoclonal antibody (M01), clone 1B7.
Lane 1: F2 transfected lysate(70 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of F2 transfected lysate using anti-F2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with F2 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged F2 is approximately 1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of F2 over-expressed 293 cell line, cotransfected with F2 Validated Chimera RNAi ( Cat # H00002147-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with F2 monoclonal antibody (M01), clone 1B7 (Cat # H00002147-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between FGA and F2. HeLa cells were stained with anti-FGA rabbit purified polyclonal 1:1200 and anti-F2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — F2
Entrez GeneID
2147GeneBank Accession#
BC051332Protein Accession#
AAH51332Gene Name
F2
Gene Alias
PT
Gene Description
coagulation factor II (thrombin)
Omim ID
176930Gene Ontology
HyperlinkGene Summary
Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. [provided by RefSeq
Other Designations
coagulation factor II|prothrombin B-chain|serine protease
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Interactome
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Pathway
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Disease
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