EPO monoclonal antibody (M02), clone 1B12
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of EPO expression in transfected 293T cell line by EPO monoclonal antibody (M02), clone 1B12.
Lane 1: EPO transfected lysate (Predicted MW: 21.3 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EPO is 0.03 ng/ml as a capture antibody.
Western Blot detection against Immunogen (47.7 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant EPO.
Immunogen
EPO (NP_000790.2, 1 a.a. ~ 193 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MGVHECPAWLWLLLSLLSLPLGLPVLGAPPRLICDSRVLERYLLEAKEAENITTGCAEHCSLNENITVPDTKVNFYAWKRMEVGQQAVEVWQGLALLSEAVLRGQALLVNSSQPWEPLQLHVDKAVSGLRSLTTLLRALGAQKEAISPPDAASAAPLRTITADTFRKLFRVYSNFLRGKLKLYTGEACRTGDR
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (80); Rat (83)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (47.7 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of EPO expression in transfected 293T cell line by EPO monoclonal antibody (M02), clone 1B12.
Lane 1: EPO transfected lysate (Predicted MW: 21.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EPO is 0.03 ng/ml as a capture antibody.ELISA
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Gene Info — EPO
Entrez GeneID
2056GeneBank Accession#
NM_000799.2Protein Accession#
NP_000790.2Gene Name
EPO
Gene Alias
EP, MGC138142
Gene Description
erythropoietin
Omim ID
133170Gene Ontology
HyperlinkGene Summary
This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq
Other Designations
epoetin
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Interactome
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Pathway
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Disease
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