This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity:
Human
Quality Control Testing:
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of ENO1 transfected lysate using mouse monoclonal anti-ENO1 and Protein A Magnetic Bead (U0007), and immunoblotted with rabbit polyclonal anti-ENO1.
Supplied Product:
Antibody pair set content: 1. Antibody pair for IP: mouse monoclonal anti-ENO1 (300 ug) 2. Antibody pair for WB: rabbit polyclonal anti-ENO1 (50 ul)
Storage Instruction:
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq
Other Designations:
2-phospho-D-glycerate hydro-lyase,MYC promoter-binding protein 1,OTTHUMP00000001706,alpha enolase like 1,enolase 1,non-neural enolase,phosphopyruvate hydratase,tau-crystallin