EIF4E 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: EIF4E transfected lysate ( 25.1 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
EIF4E transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-EIF4E full-length
Host
Human
Theoretical MW (kDa)
23.98
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-EIF4E antibody (H00001977-B01) by Western Blots.
Western Blot
Lane 1: EIF4E transfected lysate ( 25.1 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
EIF4E transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — EIF4E
Entrez GeneID
1977GeneBank Accession#
NM_001968Protein Accession#
NP_001959Gene Name
EIF4E
Gene Alias
CBP, EIF4E1, EIF4EL1, EIF4F, MGC111573
Gene Description
eukaryotic translation initiation factor 4E
Omim ID
133440Gene Ontology
HyperlinkGene Summary
All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM
Other Designations
eIF-4F 25 kDa subunit|eukaryotic translation initiation factor 4E-like 1|mRNA cap-binding protein
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Interactome
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Disease
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