DNASE2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: DNASE2 transfected lysate ( 39.6 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
DNASE2 transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DNASE2 full-length
Host
Human
Theoretical MW (kDa)
39.71
Interspecies Antigen Sequence
Mouse (72); Rat (73)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DNASE2 antibody (H00001777-B01) by Western Blots.
Western Blot
Lane 1: DNASE2 transfected lysate ( 39.6 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
DNASE2 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DNASE2
Entrez GeneID
1777GeneBank Accession#
NM_001375Protein Accession#
NP_001366Gene Name
DNASE2
Gene Alias
DNASE2A, DNL, DNL2
Gene Description
deoxyribonuclease II, lysosomal
Omim ID
126350Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the DNase family. The protein, located in the lysosome, hydrolyzes DNA under acidic conditions and mediates the breakdown of DNA during erythropoiesis and apoptosis. Two codominant alleles have been characterized, DNASE2*L (low activity) and DNASE2*H (high activity), that differ at one nucleotide in the promoter region. The DNASE2*H allele is represented in this record. [provided by RefSeq
Other Designations
DNase II alpha|DNase II, lysosomal|R31240_2|acid DNase
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Interactome
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Pathway
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Disease
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