CYB5R3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
CYB5R3 transfected lysate.
Western Blot
Lane 1: CYB5R3 transfected lysate ( 34.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CYB5R3 full-length
Host
Human
Theoretical MW (kDa)
34.2
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CYB5R3 antibody (H00001727-B01) by Western Blots.
SDS-PAGE Gel
CYB5R3 transfected lysate.
Western Blot
Lane 1: CYB5R3 transfected lysate ( 34.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CYB5R3
Entrez GeneID
1727GeneBank Accession#
NM_000398.4Protein Accession#
-Gene Name
CYB5R3
Gene Alias
B5R, DIA1
Gene Description
cytochrome b5 reductase 3
Omim ID
250800Gene Ontology
HyperlinkGene Summary
This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. These two forms are resulted from alternative splicing of the gene. Mutations in this gene cause methemoglobinemias. [provided by RefSeq
Other Designations
NADH-cytochrome b5 reductase|OTTHUMP00000028761|cytochrome b5 reductase|diaphorase (NADH) (cytochrome b-5 reductase)
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Interactome
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Pathway
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Disease
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