DDX5 MaxPab mouse polyclonal antibody (B01)
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More Files
- More Functions
Western Blot (Cell lysate)
DDX5 MaxPab polyclonal antibody. Western Blot analysis of DDX5 expression in Hela S3 NE.
Western Blot (Cell lysate)
DDX5 MaxPab polyclonal antibody. Western Blot analysis of DDX5 expression in Raw 264.7.
Western Blot (Transfected lysate)
Western Blot analysis of DDX5 expression in transfected 293T cell line (H00001655-T01) by DDX5 MaxPab polyclonal antibody.
Lane 1: DDX5 transfected lysate(67.54 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to DDX5 on HeLa cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human DDX5 protein.
Immunogen
DDX5 (AAH16027, 1 a.a. ~ 614 a.a) full-length human protein.
Sequence
MSGYSSDRDRGRDRGFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPANVMDVIARQNFTEPTAIQAQGWPVALSGLDMVGVAQTGSGKTLSYLLPAIVHINHQPFLERGDGPICLVLAPTRELAQQVQQVAAEYCRACRLKSTCIYGGAPKGPQIRDLERGVEICIATPGRLIDFLECGKTNLRRTTYLVLDEADRMLDMGFEPQIRKIVDQIRPDRQTLMWSATWPKEVRQLAEDFLKDYIHINIGALELSANHNILQIVDVCHDVEKDEKLIRLMEEIMSEKENKTIVFVETKRRCDELTRKMRRDGWPAMGIHGDKSQQERDWVLNEFKHGKAPILIATDVASRGLDVEDVKFVINYDYPNSSEDYIHRIGRTARSTKTGTAYTFFTPNNIKQVSDLISVLREANQAINPKLLQLVEDRGSGRSRGRGGMKDDRRDRYSAGKRGGFNTFRDRENYDRGYSSLLKRDFGAKTQNGVYSAANYTNGSFGSNFVSAGIQTSFRTGNPTGTYQNGYDSTQQYGSNVPNMHNGMNQQAYAYPATAAAPMIGYPMPTGYSQ
Host
Mouse
Reactivity
Human, Mouse
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
No additive
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Note
For IHC and IF applications, antibody purification with Protein A will be needed prior to use.
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Applications
Western Blot (Cell lysate)
DDX5 MaxPab polyclonal antibody. Western Blot analysis of DDX5 expression in Hela S3 NE.Western Blot (Cell lysate)
DDX5 MaxPab polyclonal antibody. Western Blot analysis of DDX5 expression in Raw 264.7.Western Blot (Transfected lysate)
Western Blot analysis of DDX5 expression in transfected 293T cell line (H00001655-T01) by DDX5 MaxPab polyclonal antibody.
Lane 1: DDX5 transfected lysate(67.54 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to DDX5 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — DDX5
Entrez GeneID
1655GeneBank Accession#
BC016027Protein Accession#
AAH16027Gene Name
DDX5
Gene Alias
DKFZp434E109, DKFZp686J01190, G17P1, HLR1, HUMP68, p68
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
Omim ID
180630Gene Ontology
HyperlinkGene Summary
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq
Other Designations
ATP-dependent RNA helicase DDX5|DEAD box-5|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD)
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Interactome
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Disease
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Publication Reference
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DDX17 promotes the production of infectious HIV-1 particles through modulating viral RNA packaging and translation frameshift.
Lorgeoux RP, Pan Q, Le Duff Y, Liang C.
Virology 2013 Sep; 443(2):384.
Application：WB-Tr, Human, HeLa cells.
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DDX17 promotes the production of infectious HIV-1 particles through modulating viral RNA packaging and translation frameshift.
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