DDX3X 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
DDX3X transfected lysate.
Western Blot
Lane 1: DDX3X transfected lysate ( 73.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DDX3X full-length
Host
Human
Theoretical MW (kDa)
73.2
Interspecies Antigen Sequence
Mouse (99)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DDX3X antibody (H00001654-B01) by Western Blots.
SDS-PAGE Gel
DDX3X transfected lysate.
Western Blot
Lane 1: DDX3X transfected lysate ( 73.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DDX3X
Entrez GeneID
1654GeneBank Accession#
NM_001356Protein Accession#
NP_001347Gene Name
DDX3X
Gene Alias
DBX, DDX14, DDX3, HLP2
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
Omim ID
300160Gene Ontology
HyperlinkGene Summary
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. This gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog. [provided by RefSeq
Other Designations
ATP-dependent RNA helicase DDX3X|CAP-Rf|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3|DEAD/H box-3|helicase like protein 2
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Interactome
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Disease
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