DDOST 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
DDOST transfected lysate.
Western Blot
Lane 1: DDOST transfected lysate ( 50.70 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DDOST full-length
Host
Human
Theoretical MW (kDa)
50.7
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DDOST antibody (H00001650-D01P) by Western Blots.
SDS-PAGE Gel
DDOST transfected lysate.
Western Blot
Lane 1: DDOST transfected lysate ( 50.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DDOST
Entrez GeneID
1650GeneBank Accession#
NM_005216.4Protein Accession#
NP_005207.2Gene Name
DDOST
Gene Alias
AGE-R1, KIAA0115, MGC2191, OK/SW-cl.45, OST, OST48, WBP1
Gene Description
dolichyl-diphosphooligosaccharide-protein glycosyltransferase
Omim ID
602202Gene Ontology
HyperlinkGene Summary
This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. [provided by RefSeq
Other Designations
OTTHUMP00000002882|advanced glycation endproduct receptor 1|dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48 kDa subunit|dolichyl-diphosphooligosaccharide-protein glycotransferase|oligosaccharyltransferase 48 kDa subunit
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Interactome
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Pathway
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Disease
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