GADD45A 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GADD45A full-length
Host
Human
Theoretical MW (kDa)
18.3
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GADD45A antibody (H00001647-B01P) by Western Blots.
SDS-PAGE Gel
GADD45A transfected lysate.
Western Blot
Lane 1: GADD45A transfected lysate ( 18.30 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GADD45A
Entrez GeneID
1647GeneBank Accession#
NM_001924Protein Accession#
AAH11757.1Gene Name
GADD45A
Gene Alias
DDIT1, GADD45
Gene Description
growth arrest and DNA-damage-inducible, alpha
Omim ID
126335Gene Ontology
HyperlinkGene Summary
This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. [provided by RefSeq
Other Designations
DNA damage-inducible transcript 1|DNA damage-inducible transcript-1|DNA-damage-inducible transcript 1|OTTHUMP00000010979|growth arrest and DNA-damage-inducible 45 alpha
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Interactome
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Pathway
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Disease
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