GADD45A monoclonal antibody (M01), clone 3D12
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant GADD45A.
Immunogen
GADD45A (AAH11757, 76 a.a. ~ 165 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
TLIQAFCCENDINILRVSNPGRLAELLLLETDAGPAASEGAEQPPDLHCVLVTNPHSSQWKDPALSQLICFCRESRYMDQWVPVINLPER
Host
Mouse
Reactivity
Human
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.53 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between MAP2K6 and GADD45A. HeLa cells were stained with anti-MAP2K6 rabbit purified polyclonal 1:1200 and anti-GADD45A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — GADD45A
Entrez GeneID
1647GeneBank Accession#
BC011757Protein Accession#
AAH11757Gene Name
GADD45A
Gene Alias
DDIT1, GADD45
Gene Description
growth arrest and DNA-damage-inducible, alpha
Omim ID
126335Gene Ontology
HyperlinkGene Summary
This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. [provided by RefSeq
Other Designations
DNA damage-inducible transcript 1|DNA damage-inducible transcript-1|DNA-damage-inducible transcript 1|OTTHUMP00000010979|growth arrest and DNA-damage-inducible 45 alpha
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Interactome
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Pathway
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Disease
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Publication Reference
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Inhibition of NF-kB activation sensitizes U937 cells to 3-azido-3-deoxythymidine induced apoptosis.
Matteucci C, Minutolo A, Balestrieri E, Marino-Merlo F, Bramanti P, Garaci E, Macchi B, Mastino A.
Cell Death & Disease 2010 Oct; 1:e81.
Application:WB-Ce, Human, U937 cells.
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Decitabine-Induced Demethylation of 5'CpG Island in GADD45A Leads to Apoptosis in Osteosarcoma Cells.
Al-Romaih K, Sadikovic B, Yoshimoto M, Wang Y, Zielenska M, Squire JA.
Neoplasia 2008 May; 10(5):471.
Application:IHC-P, WB, Human, MG63, U2OS cells , Xenograft tissue and pelleted paraffin-embedded formalin-fixed HeLa cell sections.
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Inhibition of NF-kB activation sensitizes U937 cells to 3-azido-3-deoxythymidine induced apoptosis.
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