CTH (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human CTH full-length ORF ( AAH15807, 1 a.a. - 405 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MQEKDASSQGFLPHFQHFATQAIHVGQDPEQWTSRAVVPPISLSTTFKQGAPGQHSGFEYSRSGNPTRNCLEKAVAALDGAKYCLAFASGLAATVTITHLLKAGDQIICMDDVYGGTNRYFRQVASEFGLKISFVDCSKIKLLEAAITPETKLVWIETPTNPTQKVIDIEGCAHIVHKHGDIILVVDNTFMSPYFQRPLALGADISMYSATKYMNGRSDVVMGLVSVNCESLHNRLRFLQNSLGAVPSPIDCYLCNRGLKTLHVRMEKHFKNGMAVAQFLESNPWVEKVIYPGLPSHPQHELVKRQCTGCTGMVTFYIKGTLQHAEIFLKNLKLFTLAESLGGFESLAELPAIMTHASVLKNDRDVLGISDTLIRLSVGLEDEEDLLEDLDQALKAAHPPSGSHS
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
70.07
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — CTH
Entrez GeneID
1491GeneBank Accession#
BC015807Protein Accession#
AAH15807Gene Name
CTH
Gene Alias
MGC9471
Gene Description
cystathionase (cystathionine gamma-lyase)
Gene Ontology
HyperlinkGene Summary
This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000010945|OTTHUMP00000010946|cystathionase|cysteine desulfhydrase|gamma-cystathionase|homoserine deaminase|homoserine dehydratase
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Interactome
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Pathway
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Disease
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Publication Reference
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Endogenously produced hydrogen sulfide is involved in porcine oocyte maturation in vitro.
Nevoral J, Zalmanova T, Zamostna K, Kott T, Kucerova-Chrpova V, Bodart JF, Gelaude A, Prochazka R, Orsak M, Sulc M, Klein P, Dvorakova M, Weingartova I, Vighova A, Hoskova K, Krejcova T, Jilek F, Petr J.
Nitric Oxide 2015 Dec; 51:24.
Application:IF, WB, Porcine, Oocytes.
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The Effect of Hydrogen Sulfide Donors on Lipopolysaccharide-Induced Formation of Inflammatory Mediators in Macrophages.
Whiteman M, Li L, Rose P, Tan CH, Parkinson DB, Moore PK.
Antioxidants & Redox Signaling 2010 May; 12(10):1147.
Application:Func, Buffer.
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Endogenously produced hydrogen sulfide is involved in porcine oocyte maturation in vitro.
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