CSNK1E monoclonal antibody (M07), clone 2E1
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant CSNK1E.
Immunogen
CSNK1E (NP_689407, 2 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
ELRVGNKYRLGRKIGSGSFGDIYLGANIASGEEVAIKLECVKTKHPQLHIESKFYKMMQGGVGIPSIKWCGAEGDYNVMVMELLGPSLEDLFNFCSRKF
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between TP53 and CSNK1E. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-CSNK1E mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Immunofluorescence
Immunofluorescence of monoclonal antibody to CSNK1E on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — CSNK1E
Entrez GeneID
1454GeneBank Accession#
NM_152221Protein Accession#
NP_689407Gene Name
CSNK1E
Gene Alias
HCKIE, MGC10398
Gene Description
casein kinase 1, epsilon
Omim ID
600863Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
CKI-epsilon|OTTHUMP00000063258|OTTHUMP00000063263|casein kinase 1 epsilon|casein kinase I epsilon
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Interactome
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Pathway
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Disease
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Publication Reference
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Analysis of binding interfaces of the human scaffold protein AXIN1 by peptide microarrays.
Harnoš J, Ryneš J, Víšková P, Trantírková SF, Ešner LB, Trantírek L, Bryja V.
The Journal of Biological Chemistry 2018 Oct; 293(42):16337.
Application:Peptide array, Recombinant protein.
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Analysis of binding interfaces of the human scaffold protein AXIN1 by peptide microarrays.
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