CDC2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CDC2 transfected lysate.
Western Blot
Lane 1: CDC2 transfected lysate ( 34.10 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CDC2 full-length
Host
Human
Theoretical MW (kDa)
34.1
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CDC2 antibody (H00000983-D01P) by Western Blots.
SDS-PAGE Gel
CDC2 transfected lysate.
Western Blot
Lane 1: CDC2 transfected lysate ( 34.10 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CDC2
Entrez GeneID
983GeneBank Accession#
NM_001786.2Protein Accession#
NP_001777.1Gene Name
CDC2
Gene Alias
CDC28A, CDK1, DKFZp686L20222, MGC111195
Gene Description
cell division cycle 2, G1 to S and G2 to M
Omim ID
116940Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000019660|cell cycle controller CDC2|cell division control protein 2 homolog|cell division cycle 2 protein|cyclin-dependent kinase 1|p34 protein kinase
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Interactome
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Pathway
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Disease
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