CDC2 monoclonal antibody (M01), clone 1A4-1A9
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More Files
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Western Blot (Cell lysate)
CDC2 monoclonal antibody (M01), clone 1A4-1A9. Western Blot analysis of CDC2 expression in 293.
Western Blot (Cell lysate)
CDC2 monoclonal antibody (M01), clone 1A4-1A9. Western Blot analysis of CDC2 expression in HL-60.
Western Blot (Cell lysate)
CDC2 monoclonal antibody (M01), clone 1A4-1A9 Western Blot analysis of CDC2 expression in Hela ( Cat # L013V1 ).
Western Blot (Cell lysate)
CDC2 monoclonal antibody (M01), clone 1A4-1A9. Western Blot analysis of CDC2 expression in MCF-7.
Western Blot (Cell lysate)
CDC2 monoclonal antibody (M01), clone 1A4-1A9. Western Blot analysis of CDC2 expression in Raji.
Western Blot (Cell lysate)
CDC2 monoclonal antibody (M01), clone 1A4-1A9. Western Blot analysis of CDC2 expression in Jurkat.
Western Blot (Cell lysate)
Lane 1: CDC2 monoclonal antibody (M01)(Cat # H00000983-M01) , clone 1A4-1A9. Western Blot analysis of CDC2 expression in NIH/3T3 (34.095 KDa). Lane 2: ACTB monoclonal antibody(M01)(Cat # H00000060-M01) , clone 3G4-F9 Western Blot in NIH/3T3 ( 41.73kDa ) used as specificity and loading control.
Western Blot (Cell lysate)
CDC2 monoclonal antibody (M01), clone 1A4-1A9. Western Blot analysis of CDC2 expression in A-549.
Western Blot (Transfected lysate)
Western Blot analysis of CDC2 expression in transfected 293T cell line by CDC2 monoclonal antibody (M01), clone 1A4-1A9.
Lane 1: CDC2 transfected lysate(34.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Western Blot analysis of CDC2 expression in recombinant protein by CDC2 monoclonal antibody (M01), clone 1A4-1A9.
Lane 1: CDC2 Protein (0.2 ug).
Lane 2: CDK2 Protein (0.2 ug).
Lane 3: CDK3 Protein (0.2 ug).
Lane 4: CDK4 Protein (0.2 ug).
Lane 5: CDK5 Protein (0.2 ug).
Lane 6: CDK6 Protein (0.2 ug).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CDC2 is approximately 30ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to CDC2 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (58.41 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant CDC2.
Immunogen
CDC2 (AAH14563, 1 a.a. ~ 297 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (58.41 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
CDC2 monoclonal antibody (M01), clone 1A4-1A9. Western Blot analysis of CDC2 expression in 293.Western Blot (Cell lysate)
CDC2 monoclonal antibody (M01), clone 1A4-1A9. Western Blot analysis of CDC2 expression in HL-60.Western Blot (Cell lysate)
CDC2 monoclonal antibody (M01), clone 1A4-1A9 Western Blot analysis of CDC2 expression in Hela ( Cat # L013V1 ).Western Blot (Cell lysate)
CDC2 monoclonal antibody (M01), clone 1A4-1A9. Western Blot analysis of CDC2 expression in MCF-7.Western Blot (Cell lysate)
CDC2 monoclonal antibody (M01), clone 1A4-1A9. Western Blot analysis of CDC2 expression in Raji.Western Blot (Cell lysate)
CDC2 monoclonal antibody (M01), clone 1A4-1A9. Western Blot analysis of CDC2 expression in Jurkat.Western Blot (Cell lysate)
Lane 1: CDC2 monoclonal antibody (M01)(Cat # H00000983-M01) , clone 1A4-1A9. Western Blot analysis of CDC2 expression in NIH/3T3 (34.095 KDa). Lane 2: ACTB monoclonal antibody(M01)(Cat # H00000060-M01) , clone 3G4-F9 Western Blot in NIH/3T3 ( 41.73kDa ) used as specificity and loading control.Western Blot (Cell lysate)
CDC2 monoclonal antibody (M01), clone 1A4-1A9. Western Blot analysis of CDC2 expression in A-549.Western Blot (Transfected lysate)
Western Blot analysis of CDC2 expression in transfected 293T cell line by CDC2 monoclonal antibody (M01), clone 1A4-1A9.
Lane 1: CDC2 transfected lysate(34.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Western Blot analysis of CDC2 expression in recombinant protein by CDC2 monoclonal antibody (M01), clone 1A4-1A9.
Lane 1: CDC2 Protein (0.2 ug).
Lane 2: CDK2 Protein (0.2 ug).
Lane 3: CDK3 Protein (0.2 ug).
Lane 4: CDK4 Protein (0.2 ug).
Lane 5: CDK5 Protein (0.2 ug).
Lane 6: CDK6 Protein (0.2 ug).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CDC2 is approximately 30ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to CDC2 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — CDC2
Entrez GeneID
983GeneBank Accession#
BC014563Protein Accession#
AAH14563Gene Name
CDC2
Gene Alias
CDC28A, CDK1, DKFZp686L20222, MGC111195
Gene Description
cell division cycle 2, G1 to S and G2 to M
Omim ID
116940Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000019660|cell cycle controller CDC2|cell division control protein 2 homolog|cell division cycle 2 protein|cyclin-dependent kinase 1|p34 protein kinase
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Interactome
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Pathway
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Disease
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