CD33 monoclonal antibody (M01), clone 2D12-G4
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More Files
- More Functions
Western Blot (Cell lysate)
CD33 monoclonal antibody (M01), clone 2D12-G4 Western Blot analysis of CD33 expression in HL-60 ( Cat # L014V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of CD33 expression in transfected 293T cell line by CD33 monoclonal antibody (M01), clone 2D12-G4.
Lane 1: CD33 transfected lysate(39.7 KDa).
Lane 2: Non-transfected lysate.
RNAi Knockdown (Antibody validated)
Western blot analysis of CD33 over-expressed 293 cell line, cotransfected with CD33 Validated Chimera RNAi ( Cat # H00000945-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CD33 monoclonal antibody (M01), clone 2D12-G4 (Cat # H00000945-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (65.78 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant CD33.
Immunogen
CD33 (AAH28152.1, 1 a.a. ~ 364 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MPLLLLLPLLWAGALAMDPNFWLQVQESVTVQEGLCVLVPCTFFHPIPYYDKNSPVHGYWFREGAIISGDSPVATNKLDQEVQEETQGRFRLLGDPSRNNCSLSIVDARRRDNGSYFFRMERGSTKYSYKSPQLSVHVTDLTHRPKILIPGTLEPGHSKNLTCSVSWACEQGTPPIFSWLSAAPTSLGPRTTHSSVLIITPRPQDHGTNLTCQVKFAGAGVTTERTIQLNVTYVPQNPTTGIFPGDGSGKQETRAGVVHGAIGGAGVTALLALCLCLIFFIVKTHRRKAARTAVGRNDTHPTTGSASPKHQKKSKLHGPTETSSCSGAAPTVEMDEELHYASLNFHGMNPSKDTSTEYSEVRTQ
Host
Mouse
Reactivity
Human
Isotype
IgG2a kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (65.78 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
CD33 monoclonal antibody (M01), clone 2D12-G4 Western Blot analysis of CD33 expression in HL-60 ( Cat # L014V1 ).Western Blot (Transfected lysate)
Western Blot analysis of CD33 expression in transfected 293T cell line by CD33 monoclonal antibody (M01), clone 2D12-G4.
Lane 1: CD33 transfected lysate(39.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of CD33 over-expressed 293 cell line, cotransfected with CD33 Validated Chimera RNAi ( Cat # H00000945-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CD33 monoclonal antibody (M01), clone 2D12-G4 (Cat # H00000945-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — CD33
Entrez GeneID
945GeneBank Accession#
BC028152Protein Accession#
AAH28152.1Gene Name
CD33
Gene Alias
FLJ00391, SIGLEC-3, SIGLEC3, p67
Gene Description
CD33 molecule
Omim ID
159590Gene Ontology
HyperlinkOther Designations
CD33 antigen|CD33 antigen (gp67)
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Interactome
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Pathway
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Disease
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