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Last updated: 2014/7/28
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CD33 monoclonal antibody (M01), clone 2D12-G4

  • Catalog # : H00000945-M01
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a full length recombinant CD33.
  • Immunogen:
  • CD33 (AAH28152.1, 1 a.a. ~ 364 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • MPLLLLLPLLWAGALAMDPNFWLQVQESVTVQEGLCVLVPCTFFHPIPYYDKNSPVHGYWFREGAIISGDSPVATNKLDQEVQEETQGRFRLLGDPSRNNCSLSIVDARRRDNGSYFFRMERGSTKYSYKSPQLSVHVTDLTHRPKILIPGTLEPGHSKNLTCSVSWACEQGTPPIFSWLSAAPTSLGPRTTHSSVLIITPRPQDHGTNLTCQVKFAGAGVTTERTIQLNVTYVPQNPTTGIFPGDGSGKQETRAGVVHGAIGGAGVTALLALCLCLIFFIVKTHRRKAARTAVGRNDTHPTTGSASPKHQKKSKLHGPTETSSCSGAAPTVEMDEELHYASLNFHGMNPSKDTSTEYSEVRTQ
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG2a kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00000945-M01
    Western Blot detection against Immunogen (65.78 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • CD33 monoclonal antibody (M01), clone 2D12-G4 Western Blot analysis of CD33 expression in HL-60 ( Cat # L014V1 ).
  • Protocol Download
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of CD33 expression in transfected 293T cell line by CD33 monoclonal antibody (M01), clone 2D12-G4.

    Lane 1: CD33 transfected lysate(39.7 KDa).
    Lane 2: Non-transfected lysate.
  • Protocol Download
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged CD33 is approximately 1ng/ml as a capture antibody.
  • Protocol Download
  • ELISA
  • RNAi Knockdown (Antibody validated)
  • RNAi Knockdown (Antibody validated)
  • Western blot analysis of CD33 over-expressed 293 cell line, cotransfected with CD33 Validated Chimera RNAi ( Cat # H00000945-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CD33 monoclonal antibody (M01), clone 2D12-G4 (Cat # H00000945-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
  • Protocol Download
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • ELISA
  • RNAi Knockdown (Antibody validated)
  • RNAi Knockdown (Antibody validated)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 945
  • Gene Name:
  • CD33
  • Gene Alias:
  • FLJ00391,SIGLEC-3,SIGLEC3,p67
  • Gene Description:
  • CD33 molecule
  • Other Designations:
  • CD33 antigen,CD33 antigen (gp67)
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