BUB1B 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
BUB1B transfected lysate.
Western Blot
Lane 1: BUB1B transfected lysate ( 115.61 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-BUB1B full-length
Host
Human
Theoretical MW (kDa)
115.61
Interspecies Antigen Sequence
Mouse (76)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-BUB1B antibody (H00000701-B01) by Western Blots.
SDS-PAGE Gel
BUB1B transfected lysate.
Western Blot
Lane 1: BUB1B transfected lysate ( 115.61 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — BUB1B
Entrez GeneID
701GeneBank Accession#
BC018739.1Protein Accession#
AAH18739.1Gene Name
BUB1B
Gene Alias
BUB1beta, BUBR1, Bub1A, MAD3L, SSK1, hBUBR1
Gene Description
budding uninhibited by benzimidazoles 1 homolog beta (yeast)
Gene Ontology
HyperlinkGene Summary
This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq
Other Designations
BUB1 budding uninhibited by benzimidazoles 1 homolog beta|MAD3/BUB1-related protein kinase|budding uninhibited by benzimidazoles 1, beta
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Interactome
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Pathway
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Disease
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