BUB1 purified MaxPab rabbit polyclonal antibody (D01P)
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More Files
- More Functions
Western Blot (Tissue lysate)
BUB1 MaxPab rabbit polyclonal antibody. Western Blot analysis of BUB1 expression in mouse testis.
Western Blot (Cell lysate)
BUB1 MaxPab rabbit polyclonal antibody. Western Blot analysis of BUB1 expression in Jurkat.
Western Blot (Transfected lysate)
Western Blot analysis of BUB1 expression in transfected 293T cell line (H00000699-T01) by BUB1 MaxPab polyclonal antibody.
Lane 1: BUB1 transfected lysate(122.40 KDa).
Lane 2: Non-transfected lysate.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between BUB1 and APC. HeLa cells were stained with anti-BUB1 rabbit purified polyclonal 1:1200 and anti-APC mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to BUB1 on HeLa cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Rabbit polyclonal antibody raised against a full-length human BUB1 protein.
Immunogen
BUB1 (NP_004327.1, 1 a.a. ~ 1085 a.a) full-length human protein.
Sequence
MDTPENVLQMLEAHMQSYKGNDPLGEWERYIQWVEENFPENKEYLITLLEHLMKEFLDKKKYHNDPRFISYCLKFAEYNSDLHQFFEFLYNHGIGTLSSPLYIAWAGHLEAQGELQHASAVLQRGIQNQAEPREFLQQQYRLFQTRLTETHLPAQARTSEPLHNVQVLNQMITSKSNPGNNMACISKNQGSELSGVISSACDKESNMERRVITISKSEYSVHSSLASKVDVEQVVMYCKEKLIRGESEFSFEELRAQKYNQRRKHEQWVNEDRHYMKRKEANAFEEQLLKQKMDELHKKLHQVVETSHEDLPASQERSEVNPARMGPSVGSQQELRAPCLPVTYQQTPVNMEKNPREAPPVVPPLANAISAALVSPATSQSIAPPVPLKAQTVTDSMFAVASKDAGCVNKSTHEFKPQSGAEIKEGCETHKVANTSSFHTTPNTSLGMVQATPSKVQPSPTVHTKEALGFIMNMFQAPTLPDISDDKDEWQSLDQNEDAFEAQFQKNVRSSGAWGVNKIISSLSSAFHVFEDGNKENYGLPQPKNKPTGARTFGERSVSRLPSKPKEEVPHAEEFLDDSTVWGIRCNKTLAPSPKSPGDFTSAAQLASTPFHKLPVESVHILEDKENVVAKQCTQATLDSCEENMVVPSRDGKFSPIQEKSPKQALSSHMYSASLLRLSQPAAGGVLTCEAELGVEACRLTDTDAAIAEDPPDAIAGLQAEWMQMSSLGTVDAPNFIVGNPWDDKLIFKLLSGLSKPVSSYPNTFEWQCKLPAIKPKTEFQLGSKLVYVHHLLGEGAFAQVYEATQGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMERLKPSMQHMFMKFYSAHLFQNGSVLVGELYSYGTLLNAINLYKNTPEKVMPQGLVISFAMRMLYMIEQVHDCEIIHGDIKPDNFILGNGFLEQDDEDDLSAGLALIDLGQSIDMKLFPKGTIFTAKCETSGFQCVEMLSNKPWNYQIDYFGVAATVYCMLFGTYMKVKNEGGECKPEGLFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKVFQQHYTNKIRALRNRLIVLLLECKRSRK
Host
Rabbit
Reactivity
Human, Mouse
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
BUB1 MaxPab rabbit polyclonal antibody. Western Blot analysis of BUB1 expression in mouse testis.Western Blot (Cell lysate)
BUB1 MaxPab rabbit polyclonal antibody. Western Blot analysis of BUB1 expression in Jurkat.Western Blot (Transfected lysate)
Western Blot analysis of BUB1 expression in transfected 293T cell line (H00000699-T01) by BUB1 MaxPab polyclonal antibody.
Lane 1: BUB1 transfected lysate(122.40 KDa).
Lane 2: Non-transfected lysate.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between BUB1 and APC. HeLa cells were stained with anti-BUB1 rabbit purified polyclonal 1:1200 and anti-APC mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Immunofluorescence
Immunofluorescence of purified MaxPab antibody to BUB1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — BUB1
Entrez GeneID
699GeneBank Accession#
NM_004336.2Protein Accession#
NP_004327.1Gene Name
BUB1
Gene Alias
BUB1A, BUB1L, hBUB1
Gene Description
budding uninhibited by benzimidazoles 1 homolog (yeast)
Omim ID
602452Gene Ontology
HyperlinkGene Summary
This gene encodes a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer. [provided by RefSeq
Other Designations
BUB1 budding uninhibited by benzimidazoles 1 homolog|budding uninhibited by benzimidazoles 1|mitotic spindle checkpoint kinase|putative serine/threonine-protein kinase
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Interactome
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Pathway
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Disease
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