BST2 monoclonal antibody (M15), clone 2E6
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More Files
- More Functions
Western Blot (Tissue lysate)
BST2 monoclonal antibody (M15), clone 2E6. Western Blot analysis of BST2 expression in human placenta.
Immunoprecipitation
Immunoprecipitation of BST2 transfected lysate using anti-BST2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BST2 MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged BST2 is 0.1 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to BST2 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (45.54 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant BST2.
Immunogen
BST2 (AAH33873.1, 1 a.a. ~ 180 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MASTSYDYCRVPMEDGDKRCKLLLGIGILVLLIIVILGVPLIIFTIKANSEACRDGLRAVMECRNVTHLLQQELTEAQKGFQDVEAQAATCNHTVMALMASLDAEKAQGQKKVEELEGEITTLNHKLQDASAEVERLRRENQVLSVRIADKKYYPSSQDSSSAAAPQLLIVLLGLSALLQ
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (45.54 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
BST2 monoclonal antibody (M15), clone 2E6. Western Blot analysis of BST2 expression in human placenta.Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of BST2 transfected lysate using anti-BST2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BST2 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged BST2 is 0.1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to BST2 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — BST2
Entrez GeneID
684GeneBank Accession#
BC033873Protein Accession#
AAH33873.1Gene Name
BST2
Gene Alias
CD317
Gene Description
bone marrow stromal cell antigen 2
Omim ID
600534Gene Ontology
HyperlinkGene Summary
Bone marrow stromal cells are involved in the growth and development of B-cells. The specific function of the protein encoded by the bone marrow stromal cell antigen 2 is undetermined; however, this protein may play a role in pre-B-cell growth and in rheumatoid arthritis. [provided by RefSeq
Other Designations
tetherin
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Interactome
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Disease
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Publication Reference
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Epitope Tags beside the N-Terminal Cytoplasmic Tail of Human BST-2 Alter Its Intracellular Trafficking and HIV-1 Restriction.
Lv M, Wang J, Zhang J, Zhang B, Wang X, Zhu Y, Zuo T, Liu D, Li X, Wu J, Zhang H, Yu B, Wu H, Zhao X, Kong W, Yu X.
PLoS One 2014 Oct; 9(10):e111422.
Application：Flow Cyt, IF, WB-Tr, Human, Monkey, 293T, COS-7 cells.
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Purification of eukaryotic tetherin/Vpu proteins and detection of their interaction by ELISA.
Lv M, Zhu Y, Wang J, Zhang H, Wang X, Zuo T, Liu D, Zhang J, Wu J, Kong W, Yu X.
Protein Expression and Purification 2013 Oct; 91(2):112.
Application：Det Ab, ELISA, WB, Recombinant protein.
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Polarity Changes in the Transmembrane Domain Core of HIV-1 Vpu Inhibits Its Anti-Tetherin Activity.
Lv M, Wang J, Wang X, Zuo T, Zhu Y, Kong W, Yu X.
PLoS One 2011 Jun; 6(6):e20890.
Application：Flow Cyt, Human, HeLa cells.
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Epitope Tags beside the N-Terminal Cytoplasmic Tail of Human BST-2 Alter Its Intracellular Trafficking and HIV-1 Restriction.
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