BLMH monoclonal antibody (M02), clone 4A2
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More Files
- More Functions
Western Blot (Cell lysate)
BLMH monoclonal antibody (M02), clone 4A2. Western Blot analysis of BLMH expression in K-562 ( Cat # L009V1 ).
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged BLMH is approximately 0.3ng/ml as a capture antibody.
Western Blot detection against Immunogen (36.63 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant BLMH.
Immunogen
BLMH (NP_000377, 356 a.a. ~ 454 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
NMNKAERLTFGESLMTHAMTFTAVSEKDDQDGAFTKWRVENSWGEDHGHKGYLCMTDEWFSEYVYEVVVDRKHVPEEVLAVLEQEPIILPAWDPMGALA
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
BLMH monoclonal antibody (M02), clone 4A2. Western Blot analysis of BLMH expression in K-562 ( Cat # L009V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged BLMH is approximately 0.3ng/ml as a capture antibody.ELISA
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Gene Info — BLMH
Entrez GeneID
642GeneBank Accession#
NM_000386Protein Accession#
NP_000377Gene Name
BLMH
Gene Alias
BH, BMH
Gene Description
bleomycin hydrolase
Gene Ontology
HyperlinkGene Summary
Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq
Other Designations
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Interactome
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Disease
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