ATP6V1B2 monoclonal antibody (M05), clone 2A5
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More Files
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Western Blot (Transfected lysate)
Western Blot analysis of ATP6V1B2 expression in transfected 293T cell line by ATP6V1B2 monoclonal antibody (M05), clone 2A5.
Lane 1: ATP6V1B2 transfected lysate (Predicted MW: 56.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (81.95 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant ATP6V1B2.
Immunogen
ATP6V1B2 (AAH03100, 1 a.a. ~ 511 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MALRAMRGIVNGAAPELPVPTGGPAVGAREQALAVSRNYLSQPRLTYKTVSGVNGPLVILDHVKFPRYAEIVHLTLPDGTKRSGQVLEVSGSKAVVQVFEGTSGIDAKKTSCEFTGDILRTPVSEDMLGRVFNGSGKPIDRGPVVLAEDFLDIMGQPINPQCRIYPEEMIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSNQLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFERNFIAQGPYENRTVFETLDIGWQLLRIFPKEMLKRIPQSTLSEFYPRDSAKH
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (81.95 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ATP6V1B2 expression in transfected 293T cell line by ATP6V1B2 monoclonal antibody (M05), clone 2A5.
Lane 1: ATP6V1B2 transfected lysate (Predicted MW: 56.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
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Gene Info — ATP6V1B2
Entrez GeneID
526GeneBank Accession#
BC003100Protein Accession#
AAH03100Gene Name
ATP6V1B2
Gene Alias
ATP6B1B2, ATP6B2, HO57, VATB, VPP3, Vma2
Gene Description
ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
Omim ID
606939Gene Ontology
HyperlinkGene Summary
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts. [provided by RefSeq
Other Designations
ATPase, H+ transporting, lysosomal (vacuolar proton pump), beta polypeptide, 56/58kD, isoform 2|ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B, isoform 2|H+ transporting two-sector ATPase|V-ATPase B2 subunit|endomembrane proton pump 58 kDa subu
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Interactome
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Pathway
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Disease
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